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EpiLife® Medium

Without calcium.

A sterile, liquid medium prepared without calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. This basal medium requires the addition of calcium plus an appropriate supplement prior to use. Calcium chloride is provided as a separate component with each bottle of medium. Below is the composition list. The concentrations are proprietary.

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.0confidentialn/a
L-Alanine89.0confidentialn/a
L-Arginine hydrochloride211.0confidentialn/a
L-Asparagine-H2O150.0confidentialn/a
L-Aspartic acid133.0confidentialn/a
L-Cysteine121.0confidentialn/a
L-Glutamic Acid147.0confidentialn/a
L-Glutamine146.0confidentialn/a
L-Histidine hydrochloride-H2O210.0confidentialn/a
L-Isoleucine131.0confidentialn/a
L-Leucine131.0confidentialn/a
L-Lysine hydrochloride183.0confidentialn/a
L-Methionine149.0confidentialn/a
L-Phenylalanine165.0confidentialn/a
L-Proline115.0confidentialn/a
L-Serine105.0confidentialn/a
L-Threonine119.0confidentialn/a
L-Tryptophan204.0confidentialn/a
L-Tyrosine181.0confidentialn/a
L-Valine117.0confidentialn/a
Vitamins
Choline chloride140.0confidentialn/a
D-Pantothenic Acid238.0confidentialn/a
Folic Acid441.0confidentialn/a
Myo-Inositol180.0confidentialn/a
Niacinamide122.0confidentialn/a
Pyridoxal hydrochloride206.0confidentialn/a
Riboflavin376.0confidentialn/a
Thiamine hydrochloride337.0confidentialn/a
Vitamin B121355.0confidentialn/a
d-Biotin244.0confidentialn/a
Inorganic Salts
Ammonium Molybdate ((NH4)6Mo7O24-4H2O)1236.0confidentialn/a
Ammonium metavanadate (NH4VO3)117.0confidentialn/a
Cupric sulfate (CuSO4-5H2O)250.0confidentialn/a
Ferric sulfate (FeSO4-7H2O)278.0confidentialn/a
Magnesium Chloride (MgCl2-6H2O)203.0confidentialn/a
Manganese Sulfate (MnSO4-H20)169.0confidentialn/a
Nickelous Chloride NiCl2 6H2O238.0confidentialn/a
Potassium Chloride (KCl)75.0confidentialn/a
Sodium Bicarbonate (NaHCO3)84.0confidentialn/a
Sodium Chloride (NaCl)58.0confidentialn/a
Sodium Meta Silicate Na2SiO3 9H2O284.0confidentialn/a
Sodium Phosphate dibasic (Na2HPO4-7H2O)268.0confidentialn/a
Sodium Selenite (Na2SeO3)173.0confidentialn/a
Tin Chloride (SnCl2.2H2O)226.0confidentialn/a
Zinc sulfate (ZnSO4-7H2O)288.0confidentialn/a
Other Components
Adenine.HCl172.0confidentialn/a
D-Glucose (Dextrose)180.0confidentialn/a
DL-alpha-Lipoic Acid206.0confidentialn/a
Ethanolamine61.0confidentialn/a
HEPES238.0confidentialn/a
O-Phosphorylethanolamine141.0confidentialn/a
Phenol Red376.4confidentialn/a
Putrescine 2HCl161.0confidentialn/a
Sodium Pyruvate110.0confidentialn/a
Thymidine242.0confidentialn/a

Reference:

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