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引用和文献 (9)
Gibco™
Bac-to-Bac™ HT 载体试剂盒
Bac-to-Bac™ HT 载体设计作为 Bac-to-Bac™ 杆状病毒表达系统的一部分使用（货号：10359-016），用于在大肠杆菌中生成杆状病毒质粒后在 Sf9、Sf21 或了解更多信息
| 货号 | 数量 |
|---|---|
| 10584027 | 1 kit |
货号 10584027
价格（CNY)
14,997.00
Each
数量:
1 kit
价格（CNY)
14,997.00
Each
Bac-to-Bac™ HT 载体设计作为 Bac-to-Bac™ 杆状病毒表达系统的一部分使用（货号：10359-016），用于在大肠杆菌中生成杆状病毒质粒后在 Sf9、Sf21 或 High Five™ 细胞中表达和纯化组氨酸标记的重组蛋白。pFastBac™ HT 载体具有以下特性：
•强多角体蛋白启动子用于蛋白表达
• 三个读码框以简化克隆
• N 端 6xHis 标记可对重组融合蛋白进行简单纯化
• TEV 蛋白酶切割位点用于在蛋白纯化后去除组氨酸标记
•强多角体蛋白启动子用于蛋白表达
• 三个读码框以简化克隆
• N 端 6xHis 标记可对重组融合蛋白进行简单纯化
• TEV 蛋白酶切割位点用于在蛋白纯化后去除组氨酸标记
仅供科研使用。不可用于诊断程序。
规格
切割TEV 蛋白酶识别位点
产品类型载体试剂盒
数量1 kit
载体pFastBac
克隆方法限制性内切酶/MCS
产品线Bac-to-Bac
促进剂多角体蛋白
蛋白标记His 标签 (6x)
Unit SizeEach
内容与储存
Bac-to-Bac™ HT 载体试剂盒包含 10 µg pFastBac™ HT 载体和 pFastBac HT-CAT 对照载体。
载体在 -20°C 下储存。妥善储存时，所有组分可保证稳定储存 6 个月。
载体在 -20°C 下储存。妥善储存时，所有组分可保证稳定储存 6 个月。
常见问题解答 (FAQ)
白色菌落不能在液体培养基中生长。我该怎么办？
为什么会出现蓝色菌落？为什么会出现中心为蓝色而边缘为白色的菌落？
我得到的主要是白色/野生型空斑，而不是蓝色/重组型空斑。哪里出错了？
感染细胞后，我在一个细胞中看到较大的多角体，而在相邻细胞中看到较小的多角体（数量更多）。这正常吗？
我担心得不到空斑。出现空斑需要几天？空斑通常为多大？
引用和文献 (9)
引用和文献
Abstract
Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin.
Journal:J Biol Chem
PubMed ID:11413130
'PAK4 is the most recently identified member of the PAK family of serine/threonine kinases. PAK4 differs from other members of the PAK family in sequence and in many of its functions. Previously, we have shown that an important function of this kinase is to mediate the induction of filopodia in
Sp3 Represses Gene Expression via the Titration of Promoter-specific Transcription Factors.
Journal:J Biol Chem
PubMed ID:11773047
'We have determined previously that Sp3 encodes three distinct gene products as follows: a full-length protein (Sp3) that is an activator of transcription and two isoforms (M1 and M2) derived via internal translational initiation that function as transcriptional repressors. To identify amino acids and functions required for transcriptional repression, we
Identification of phosphorylated residues that affect the activity of the mitotic kinase Aurora-A.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12422018
'The activity of the kinase Aurora-A (Aur-A) peaks during mitosis and depends on phosphorylation by one or more unknown kinases. Mitotic phosphorylation sites were mapped by mass spec sequencing of recombinant Aur-A protein that had been activated by incubation in extracts of metaphase-arrested Xenopus eggs. Three sites were identified: serine
15-Lipoxygenase metabolism of 2-arachidonylglycerol. Generation of a peroxisome proliferator-activated receptor alpha agonist.
Journal:J Biol Chem
PubMed ID:11956198
'The recent demonstrations that cyclooxygenase-2 and leukocyte-type 12-lipoxygenase (LOX) efficiently oxygenate 2-arachidonylglycerol (2-AG) prompted an investigation into related oxygenases capable of metabolizing this endogenous cannabinoid receptor ligand. We evaluated the ability of six LOXs to catalyze the hydroperoxidation of 2-AG. Soybean 15-LOX, rabbit reticulocyte 15-LOX, human 15-LOX-1, and human 15-LOX-2
Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing.
Journal:Nat Biotechnol
PubMed ID:12592410
RNA interference (RNAi) is a powerful method for specifically silencing gene expression in diverse cell types. RNAi is mediated by approximately 21-nucleotide small interfering RNAs (siRNAs), which are produced from larger double-stranded RNAs (dsRNAs) in vivo through the action of Dicer, an RNase III-family enzyme. Transfecting cells with siRNAs rather