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Invitrogen
IL-10 Monoclonal Antibody (JES3-9D7), PE, eBioscience™
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FIGURE: 1 / 10
IL-10 Antibody (12-7108-82) in Flow
Normal human peripheral blood cells were stimulated with PMA and Ionomycin in the presence of Brefeldin A for 5 hours. The cells were surface stained with Anti-Human CD4 FITC (Product # 11-0049-42) and intracellularly stained with Anti-Human IL-10 PE.
Product Details
12-7108-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Fungi,
Human,
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
JES3-9D7
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).
Applications Reported:JES3-9D7 has been reported for use in intracellular staining and flow cytometric analysis.
Applications Tested: Has been tested by intracellular staining and flow cytometric analysis of human activated cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor
Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF
UniProt ID: (Human) P22301
Entrez Gene ID: (Human) 3586
response to molecule of bacterial origin
negative regulation of cytokine secretion involved in immune response
negative regulation of chronic inflammatory response to antigenic stimulus
positive regulation of B cell apoptotic process
inflammatory response
cytoplasmic sequestering of NF-kappaB
cell-cell signaling
aging
regulation of gene expression
response to activity
response to inactivity
hemopoiesis
B cell differentiation
leukocyte chemotaxis
negative regulation of myeloid dendritic cell activation
negative regulation of B cell proliferation
negative regulation of interferon-gamma production
negative regulation of interleukin-1 production
negative regulation of interleukin-12 production
negative regulation of interleukin-18 production
negative regulation of interleukin-6 production
negative regulation of interleukin-8 production
negative regulation of tumor necrosis factor production
receptor biosynthetic process
response to insulin
response to carbon monoxide
cellular response to hepatocyte growth factor stimulus
type 2 immune response
B cell proliferation
negative regulation of T cell proliferation
response to drug
negative regulation of tumor necrosis factor biosynthetic process
defense response to bacterium
defense response to protozoan
negative regulation of apoptotic process
negative regulation of growth of symbiont in host
negative regulation of nitric oxide biosynthetic process
regulation of isotype switching
negative regulation of MHC class II biosynthetic process
positive regulation of MHC class II biosynthetic process
negative regulation of interferon-alpha biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
positive regulation of cytokine secretion
negative regulation of membrane protein ectodomain proteolysis
positive regulation of sequence-specific DNA binding transcription factor activity
response to glucocorticoid
regulation of sensory perception of pain
negative regulation of cytokine activity
branching involved in labyrinthine layer morphogenesis
cellular response to lipopolysaccharide
cellular response to estradiol stimulus
negative regulation of chemokine (C-C motif) ligand 5 production
regulation of complement-dependent cytotoxicity
negative regulation of endothelial cell apoptotic process
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