Invitrogen

CD31 (PECAM-1) Monoclonal Antibody (TLD-3A12), Biotin, eBioscience™

Name: CD31 (PECAM-1) Monoclonal Antibody (TLD-3A12), Biotin, eBioscience\u2122
Brand: Invitrogen
SKU: 13-0310-82

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Additional Information:

抗体检测数据 (1)
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CD31 (PECAM-1) Antibody in Flow Cytometry (Flow)

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CD31 (PECAM-1) Antibody (13-0310-82) in Flow

Staining of rat splenocytes with Anti-Rat CD45R (B220) PE (Product # 12-0460-82) and 0.125 µg of Mouse IgG1 K Isotype Control Biotin (Product # 13-4714-85) (left) or 0.125 µg of Anti-Rat CD31 (PECAM-1) Biotin (right) followed by Streptavidin APC (Product # 17-4314-82). Total viable cells were used for analysis.

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产品信息

13-0310-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

TLD-3A12

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2572763

产品详细信息

Description: The TLD-3A12 monoclonal antibody reacts with rat CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-kDa surface protein is expressed highly by endothelial cells but at low levels on all leukocytes and platelets. Studies have demonstrated that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interactions, and signal transduction.

Applications Reported: This TLD-3A12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TLD-3A12 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

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