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Invitrogen
CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Biotin, eBioscience™
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-Flow-20170216130641.jpg?time=20220826 "CD274 (PD-L1, B7-H1) Antibody in Flow Cytometry (Flow)")
图: 1 / 64
CD274 (PD-L1, B7-H1) Antibody (13-5982-82) in Flow
Staining of C57Bl/6 splenocytes with 0.125 µg of Rat IgG2a Isotype Control Biotin (Product # 13-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD274 (B7-H1) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
-Flow-20170216130641.jpg?time=20220826)
-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD274 (PD-L1, B7-H1) Antibody (13-5982-82) in Flow")
产品信息
13-5982-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Rat
/ IgG2a, lambda
分类
Monoclonal
类型
Antibody
克隆号
MIH5
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.
Applications Reported: The MIH5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1
基因别名: A530045L16Rik; B7h1; Cd274; Pdcd1l1; Pdcd1lg1; Pdl1
UniProt ID: (Mouse) Q9EP73
Entrez Gene ID: (Mouse) 60533
immune response
signal transduction
cell surface receptor signaling pathway
positive regulation of cell migration
T cell costimulation
negative regulation of interferon-gamma production
negative regulation of interleukin-10 production
positive regulation of T cell proliferation
negative regulation of T cell proliferation
negative regulation of activated T cell proliferation
toxin transport
negative regulation of tumor necrosis factor superfamily cytokine production
positive regulation of interleukin-10 secretion
