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Invitrogen
ALDH1L1 Monoclonal Antibody (7G8), Biotin, eBioscience™
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FIGURE: 1 / 2
ALDH1L1 Antibody (13-9595-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human cerebellum using 20 µg/mL Mouse IgG2b K Isotype Control Biotin (left) or 20 µg/mL Anti-Human 10-formyltetrahydrofolate dehydrogenase (ALDH1L1) Biotin (right) followed by streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.
Product Details
13-9595-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
7G8
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 7G8 monoclonal antibody reacts with human 10-formyltetrahydrofolate dehydrogenase (ALDH1L1) a protein involved in folate metabolism. The cytosolic enzyme, with homology to the aldehyde dehydrogenase (ALDH) family, is involved in the conversion of 10-formyltetrahydrofolate, NADP and water to tetrahydrofolate, NADPH and carbon dioxide. Folate metabolism is essential for cell growth and proliferation and decreased levels of folate have been implicated in neural tube defects in addition to increased cancer risk. This isoform (1L1) is expressed in glial cells has been identified as a highly specific marker for astrocytes labeling both the cell body and processes.
Applications Reported: This 7G8 antibody has been reported for use in flow cytometric analysis, western blotting, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This 7G8 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue with low pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 10-formyltetrahydrofolate dehydrogenase; 10-FTHFDH; 10fTHF; 10FTHFDH; aldehyde dehydrogenase 1 family, member L1; Aldehyde dehydrogenase family 1 member L1; Cytosolic 10-formyltetrahydrofolate dehydrogenase; DKFZp781N0997; MER57B-ALDH1L1
Gene Aliases: 10-fTHF; 10-FTHFDH; ALDH1L1; FDH; FTHFD
UniProt ID: (Human) O75891
Entrez Gene ID: (Human) 10840
catalytic activity
aldehyde dehydrogenase (NAD) activity
formyltetrahydrofolate dehydrogenase activity
hydroxymethyl-, formyl- and related transferase activity
aminobutyraldehyde dehydrogenase activity
4-trimethylammoniobutyraldehyde dehydrogenase activity
dehydrogenase
RNA processing factor
oxidoreductase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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