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Invitrogen
CD106 (VCAM-1) Monoclonal Antibody (eBioMR106 (MR106)), eBioscience™
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-Flow-20170216130641.jpg?time=20220826 "CD106 (VCAM-1) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 2
CD106 (VCAM-1) Antibody (14-1060-82) in Flow
Staining of rat bone marrow cells with 0.25 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Rat CD106 (VCAM-1) Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87). Cells in the large scatter population were used for analysis.
-Flow-20170216130641.jpg?time=20220826)
-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD106 (VCAM-1) Antibody (14-1060-82) in Flow")
Product Details
14-1060-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
C. elegans,
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
eBioMR106 (MR106)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The eBioMR106 monoclonal antibody reacts with rat CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and further upregulate VCAM-1 upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.
Applications Reported: This eBioMR106 (MR106) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections.
Applications Tested: This eBioMR106 (MR106) antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD106; DKFZp779G2333; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1; VCAM-1
Gene Aliases: Vcam-1; Vcam1; VCAM1B
UniProt ID: (Rat) P29534
Entrez Gene ID: (Rat) 25361
response to hypoxia
acute inflammatory response
chronic inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
heart development
aging
response to nutrient
amine metabolic process
response to zinc ion
response to ionizing radiation
viral process
membrane to membrane docking
response to lipopolysaccharide
response to nicotine
cellular response to vascular endothelial growth factor stimulus
positive regulation of T cell proliferation
response to ethanol
leukocyte tethering or rolling
oxidation-reduction process
cell chemotaxis
embryonic placenta morphogenesis
chorio-allantoic fusion
cellular response to glucose stimulus
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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