Invitrogen

EOMES Monoclonal Antibody (Dan11mag), eBioscience™

Name: EOMES Monoclonal Antibody (Dan11mag), eBioscience\u2122
Brand: Invitrogen
SKU: 14-4875-82

59个已发表图片

125 篇参考文献

查看其他34个EOMES抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (59)

EOMES Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 60

EOMES Antibody (14-4875-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded mouse spleen using 5 µg/mL Anti-Mouse EOMES Purified followed by Anti-Rat IgG Biotin, Streptavidin HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.

EOMES Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

EOMES Antibody in Immunocytochemistry (ICC/IF)

EOMES Antibody in Immunohistochemistry (Frozen) (IHC (F))

EOMES Antibody in Immunohistochemistry (IHC)

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产品信息

14-4875-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看50篇已发表文章 50篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫组化（冰冻） (IHC (F))

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

查看11篇已发表文章 11篇已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看58篇已发表文章 58篇已发表文章

CUT&RUN (C&R)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Bat, C. elegans, Human, Mouse, Rat

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

Dan11mag

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11042577

产品详细信息

Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This Dan11mag antibody has been reported for use in immunohistochemistry of formalin-fixed paraffin embedded tissue sections, intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated Dan11mag is recommended for use in intracellular flow cytometry.).

Applications Tested: This Dan11mag antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse splenocytes and/or western blotting at 5 µg/mL. This Dan11mag antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low pH antigen retrieval at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

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