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Invitrogen
EOMES Monoclonal Antibody (WD1928), eBioscience™
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FIGURE: 1 / 20
EOMES Antibody (14-4877-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human thymus using 5 µg/mL Mouse IgG1 K Isotype Control Purified (left) or 5 µg/mL Anti-Human EOMES Purified (right) followed by Anti-Mouse IgG Biotin, Avidin HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.
Product Details
14-4877-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Pig
Published species
C. elegans,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
WD1928
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This WD1928 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.
Applications Reported: This WD1928 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This WD1928 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2
Gene Aliases: EOMES; TBR2
UniProt ID: (Human) O95936
Entrez Gene ID: (Human) 8320, (Pig) 100622125
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II activating transcription factor binding
transcriptional repressor activity, RNA polymerase II transcription factor binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
endoderm formation
mesoderm formation
endodermal cell fate specification
trophectodermal cell differentiation
adaptive immune response
CD8-positive, alpha-beta T cell differentiation involved in immune response
transcription, DNA-templated
brain development
cardioblast differentiation
stem cell population maintenance
olfactory bulb development
cerebral cortex regionalization
cerebral cortex neuron differentiation
interferon-gamma production
skeletal muscle cell differentiation
positive regulation of cell differentiation
regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell differentiation involved in embryonic placenta development
mesodermal to mesenchymal transition involved in gastrulation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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