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CD301b (MGL2) Monoclonal Antibody (11A10-B7), APC, eBioscience™
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FIGURE: 1 / 1
CD301b (MGL2) Antibody (17-3011-82) in Flow
C57BL/6 mouse skin draining lymph node cells were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0032-82), CD19 Monoclonal Antibody, FITC (Product # 11-0193-82), MHC Class II (I-A/I-E) Monoclonal Antibody, eFluor 450 (Product # 48-5321-82), CD11c Monoclonal Antibody, PE (Product # 12-0114-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, APC (Product # 17-4321-81) (left) or 0.125 µg of CD301b (MGL2) Monoclonal Antibody, APC (right). Viable cells, as determined by 7-AAD (Product # 00-6993-50), that were CD19-, CD3-, MHC II... View More
Product Details
17-3011-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, lambda
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
11A10-B7
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Description
The monoclonal antibody 11A10-B7 recognizes mouse CD301b. CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2). CD301b expression is restricted to dermal dendritic cells, connection tissue, and bone marrow derived dendritic cells. Macrophages also express CD301b at low levels. CD301b contains a single carbohydrate recognition domain that recognizes protein antigens that have a galactose and N-acetylgalactosamine (Gal/GalNAc). Gal/GalNAc protein antigens are efficiently internalized through CD301b and presented to antigen specific CD4+ cells via MHC Class II. CD301b+ dendritic cells are believed to play an important role in TH2 priming.
Applications Tested
This 11A10-B7 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 0.25 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the BestProtocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols®Section under the Resources tab online.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Target Information
CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2). CD301b expression is restricted to dermal dendritic cells, connection tissue, and bone marrow derived dendritic cells. Macrophages also express CD301b at low levels. CD301b contains a single carbohydrate recognition domain that recognizes protein antigens that have a galactose and N-acetylgalactosamine (Gal/GalNAc). Gal/GalNAc protein antigens are efficiently internalized through CD301b and presented to antigen specific CD4+ cells via MHC Class II. CD301b+ dendritic cells are believed to play an important role in TH2 priming.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD301; macrophage galactose N-acetyl-galactosamine specific lectin 2; macrophage galactose-type C-type lectin 2
Gene Aliases: CD301b
Entrez Gene ID: (Mouse) 216864
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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