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Invitrogen
Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™
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产品信息
25-4031-82
应用
建议稀释比
流式细胞分析 (Flow)
Assay-Dependent
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Rat
/ IgG2b, kappa
分类
Monoclonal
类型
Isotype Control
克隆号
eB149/10H5
偶联物
激发/发射光谱
569/780 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The rat IgG2b monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported: PE-Cy7 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.
Applications Tested: Rat IgG2b K Isotype Control PE-Cyanine7 has been tested by flow cytometric analysis of mouse splenocytes. It should be used at the same concentration as the experimental antibody.
Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
