Invitrogen

Jagged 2 Monoclonal Antibody (HMJ2-1), eFluor™ 660, eBioscience™

Name: Jagged 2 Monoclonal Antibody (HMJ2-1), eFluor\u2122 660, eBioscience\u2122
Brand: Invitrogen
SKU: 50-3392-82

查看其他22个Jagged 2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Jagged 2 Antibody in Flow Cytometry (Flow)

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Jagged 2 Antibody (50-3392-82) in Flow

Staining of C57Bl/6 splenocytes with 0.5 µg of Armenian Hamster IgG Isotype Control eFluor® 660 (Product # 50-4888-82) (blue histogram) or 0.5 µg of Anti-Mouse Jagged 2 eFluor® 660 (purple histogram). CD8+CD11c+ cells were used for analysis.

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产品信息

50-3392-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

产品规格

种属反应

Mouse, Rat

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

HMJ2-1

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574203

产品详细信息

Description: This HMJ2-1 monoclonal antibody reacts with mouse Jagged-2, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g., Notch 1-4), Jagged-2 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Jagged-2 is expressed by thymic lymphoid and stromal cells, as well as macrophages and dendritic cells in the spleen.

Note: Staining of endogenous splenic dendritic (CD8+CD11c+) cells could only be consistently confirmed in cells isolated from C57BL/6, but not Balb/c, mice. Other strains have not been tested. The antiobdy has been reported to crossreact to rat and have blocking function.

Applications Reported: This HMJ2-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HMJ2-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. JAG2 is one of several ligands that activate Notch and related receptors.

仅用于科研。不用于诊断过程。未经明确授权不得转售。