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          • Primary Antibodies ›
          • beta Catenin Antibodies

          Invitrogen

          beta Catenin Monoclonal Antibody (15B8), Alexa Fluor™ 488, eBioscience™

          11 Published Figures
          11 References
          View all (128) beta Catenin antibodies

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          Cite beta Catenin Monoclonal Antibody (15B8), Alexa Fluor™ 488, eBioscience™

          Additional Information:
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          • Antibody Testing Data (5)
          • Published Figures (11)
          beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 16

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          beta Catenin Antibody (53-2567-42) in IHC (P)

          Immunohistochemical analysis of Beta Catenin was performed using formalin-fixed paraffin-embedded human colon adenocarcinoma tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsiu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta Catenin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta Catenin Antibody in Flow Cytometry (Flow)
          beta Catenin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          beta Catenin Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)
          beta Catenin Antibody in Immunocytochemistry (ICC/IF)
          beta Catenin Antibody in Western Blot (WB)
          beta Catenin Antibody in Western Blot, Immunoprecipitation (WB, IP)
          beta Catenin Antibody in Western Blot (WB)
          beta Catenin Antibody in Western Blot (WB)
          beta Catenin Antibody in Western Blot (WB)
          beta Catenin Antibody in Flow Cytometry (Flow)
          beta Catenin Antibody in Flow Cytometry (Flow)
          beta Catenin Antibody in Flow Cytometry (Flow)

          Product Details

          53-2567-42

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          5 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          -
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          5 µL (0.25 µg)/test
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          15B8

          Conjugate

          Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488
          • Unconjugated
          • eFluor 660
          • PE 
          • Request custom conjugation

          Excitation/Emission Max

          499/520 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10804034

          Product Specific Information

          Description: The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt and beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.

          Applications Reported: This 15B8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

          Applications Tested: This 15B8 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cell line using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: Bcatenin; beta 1 88kDa; Beta-catenin; Betacatenin; C20orf33; Cadherin associated protein; catenin; catenin (cadherin-associated protein), beta 1, 88kDa; Catenin b 1; Catenin b1; Catenin beta-1; Catenin beta1; Catenin β 1; Catenin β1; CTNB1; dJ633O20.1; DKFZp686D02253; FLJ25606; FLJ37923; NAP; NYD-SP19; OTTHUMP00000209289; P14L; PP8304; RP5-1118M15.1; unnamed protein product; β catenin; βcatenin

          View more View less

          Gene Aliases: armadillo; Bfc; Catnb; CTNNB; CTNNB1; EVR7; Mesc; MRD19; NEDSDV; OK/SW-cl.35; PRO2286

          View more View less

          UniProt ID: (Human) P35222, (Mouse) Q02248

          View more View less

          Entrez Gene ID: (Human) 1499, (Mouse) 12387

          View more View less

          Function(s)
          transcription cofactor binding transcription corepressor binding chromatin binding transcription coactivator activity receptor binding protein binding ligand-dependent nuclear receptor binding enzyme binding kinase binding protein kinase binding phosphatase binding protein phosphatase binding estrogen receptor binding ubiquitin protein ligase binding ion channel binding alpha-catenin binding cadherin binding SMAD binding RNA polymerase II sequence-specific DNA binding transcription factor binding I-SMAD binding disordered domain specific binding DNA-binding transcription factor binding histone methyltransferase binding RNA polymerase II activating transcription factor binding DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding signal transducer activity protein C-terminus binding transcription factor binding protein complex binding ionotropic glutamate receptor binding nuclear hormone receptor binding transcription regulatory region DNA binding nitric-oxide synthase binding R-SMAD binding repressing transcription factor binding euchromatin binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter embryonic axis specification cell morphogenesis involved in differentiation skeletal system development patterning of blood vessels vasculogenesis branching involved in ureteric bud morphogenesis in utero embryonic development gastrulation with mouth forming second endoderm formation cell fate specification cell fate determination endodermal cell fate commitment neuron migration kidney development neural plate development vasculature development positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation lens morphogenesis in camera-type eye ventricular compact myocardium morphogenesis regulation of secondary heart field cardioblast proliferation mesenchymal to epithelial transition involved in metanephros morphogenesis metanephros morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter cell adhesion cell-matrix adhesion chemical synaptic transmission ectoderm development nervous system development glial cell fate determination heart development cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation anterior/posterior axis specification dorsal/ventral axis specification dorsal/ventral pattern formation proximal/distal pattern formation cellular process regulation of gene expression positive regulation of gene expression positive regulation of epithelial to mesenchymal transition positive regulation of heparan sulfate proteoglycan biosynthetic process Schwann cell proliferation Wnt signaling pathway morphogenesis of embryonic epithelium single organismal cell-cell adhesion stem cell population maintenance layer formation in cerebral cortex central nervous system vasculogenesis hair cycle process hemopoiesis cell differentiation T cell differentiation osteoclast differentiation lung development male genitalia development regulation of epithelial cell differentiation positive regulation of epithelial cell differentiation forebrain development regulation of centriole-centriole cohesion pancreas development hair follicle morphogenesis regulation of myelination positive regulation of telomere maintenance via telomerase negative regulation of chondrocyte differentiation response to estradiol T cell differentiation in thymus negative regulation of protein sumoylation response to cytokine adherens junction organization adherens junction assembly protein localization to cell surface cellular protein localization embryonic heart tube development genitalia morphogenesis embryonic forelimb morphogenesis embryonic hindlimb morphogenesis embryonic skeletal limb joint morphogenesis regulation of cell proliferation regulation of T cell proliferation odontogenesis of dentin-containing tooth regulation of cell fate specification embryonic digit morphogenesis regulation of apoptotic process positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade skin development response to estrogen canonical Wnt signaling pathway involved in positive regulation of epithelial to mesenchymal transition canonical Wnt signaling pathway involved in negative regulation of apoptotic process bone resorption regulation of cell differentiation negative regulation of cell differentiation positive regulation of endothelial cell differentiation regulation of osteoblast differentiation positive regulation of osteoblast differentiation regulation of osteoclast differentiation negative regulation of osteoclast differentiation positive regulation of fibroblast growth factor receptor signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of mitotic cell cycle, embryonic chromatin-mediated maintenance of transcription cell maturation synaptic vesicle transport animal organ development thymus development oocyte development embryonic foregut morphogenesis regulation of smooth muscle cell proliferation negative regulation of oligodendrocyte differentiation synapse organization smooth muscle cell differentiation protein heterooligomerization positive regulation of telomerase activity cardiac muscle cell proliferation oviduct development canonical Wnt signaling pathway limb development trachea morphogenesis trachea formation epithelial tube branching involved in lung morphogenesis lung cell differentiation lung-associated mesenchyme development mesenchyme development lung induction epithelial cell differentiation involved in prostate gland development positive regulation of epithelial cell proliferation involved in prostate gland development hair follicle placode formation mesenchymal cell proliferation involved in lung development cardiac vascular smooth muscle cell differentiation coronary artery morphogenesis epicardium-derived cardiac vascular smooth muscle cell differentiation positive regulation of branching involved in lung morphogenesis endothelial tube morphogenesis fungiform papilla formation canonical Wnt signaling pathway involved in positive regulation of cardiac outflow tract cell proliferation sympathetic ganglion development regulation of centromeric sister chromatid cohesion cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to organic cyclic compound cellular response to indole-3-methanol renal system development renal vesicle formation renal inner medulla development renal outer medulla development nephron tubule formation mesenchyme morphogenesis regulation of nephron tubule epithelial cell differentiation regulation of calcium ion import negative regulation of neuron death cellular response to insulin-like growth factor stimulus regulation of protein localization to cell surface positive regulation of determination of dorsal identity negative regulation of apoptotic signaling pathway MAPK cascade protein polyubiquitination cell morphogenesis osteoblast differentiation epithelial to mesenchymal transition tissue homeostasis chondrocyte differentiation outflow tract morphogenesis negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis transcription from RNA polymerase II promoter epidermal growth factor receptor signaling pathway neuroblast proliferation protein localization fibroblast growth factor receptor signaling pathway response to xenobiotic stimulus mesenchymal cell proliferation gene expression negative regulation of gene expression regulation of epithelial to mesenchymal transition negative regulation of angiogenesis hypothalamus development neuron differentiation epithelial cell differentiation midbrain development hindbrain development regulation of protein ubiquitination positive regulation of transcription elongation from RNA polymerase II promoter positive regulation of homotypic cell-cell adhesion hair cell differentiation detection of muscle stretch astrocyte-dopaminergic neuron signaling negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process cell-cell adhesion mediated by cadherin canonical Wnt signaling pathway involved in mesenchymal stem cell differentiation positive regulation of cell differentiation regulation of angiogenesis positive regulation of angiogenesis regulation of fibroblast proliferation embryonic organ development positive regulation of skeletal muscle tissue development neuron fate determination oligodendrocyte differentiation regulation of neurogenesis negative regulation of neurogenesis detection of mechanical stimulus myoblast proliferation regulation of timing of anagen regulation of synapse assembly mesenchymal to epithelial transition lung epithelial cell differentiation epithelial cell proliferation involved in prostate gland development establishment of blood-brain barrier cranial ganglion development stem cell proliferation mesenchymal stem cell differentiation negative regulation of canonical Wnt signaling pathway acinar cell differentiation synaptic vesicle clustering apoptotic signaling pathway cell-cell adhesion positive regulation of odontoblast differentiation negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway cranial skeletal system development midbrain dopaminergic neuron differentiation positive regulation of blood vessel branching neuron projection extension embryonic brain development dorsal root ganglion development establishment of blood-retinal barrier positive regulation of neural precursor cell proliferation positive regulation of myoblast proliferation positive regulation of stem cell proliferation
          It has to be done as per old AB suggested Products section.

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