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Invitrogen
Glucagon Recombinant Mouse Monoclonal Antibody (ICACLS)
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FIGURE: 1 / 4
Glucagon Antibody (740062M) in ICC/IF
Immunofluorescence analysis of Glucagon was performed using Glucagon Recombinant Mouse Monoclonal Antibody (ICACLS) (Product # 740062M) on 70-80% confluent PANC-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with Glucagon Recombinant Mouse Monoclonal Antibody (ICACLS) (Product # 740062M, 1:400) in 0.1% BSA, incubated at 4 degree Celsius overnight, followed by labelling with Goat anti-Mou... View More
Product Details
740062M
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
ExpiCHO
Class
Recombinant Monoclonal
Type
Antibody
Clone
ICACLS
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogensis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide residue almost exclusively with the exsistence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in qualification of the hormone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glicentin-related polypeptide; GLP1/2; glucagon-like peptide 1; glucagon-like peptide 2; IL12p35; IL12P40; OXY; preproglucagon; Pro-glucagon
Gene Aliases: GCG; GLP-1; GLP1; GLP2; GRPP
UniProt ID: (Human) P01275
Entrez Gene ID: (Human) 2641
gluconeogenesis
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
feeding behavior
protein kinase A signaling
response to activity
positive regulation of insulin secretion involved in cellular response to glucose stimulus
glucose homeostasis
negative regulation of apoptotic process
positive regulation of gluconeogenesis
regulation of insulin secretion
positive regulation of ERK1 and ERK2 cascade
cellular response to glucagon stimulus
positive regulation of calcium ion import
negative regulation of execution phase of apoptosis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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