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IRF8 Recombinant Mouse Monoclonal Antibody (GW4CML3), Alexa Fluor™ Plus 647
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FIGURE: 1 / 3
IRF8 Antibody (740242MP647) in WB
Western blot was performed using IRF8 Recombinant Mouse Monoclonal Antibody (GW4CML3), Alexa Fluor™ Plus 647 (Product # 740242MP647) and ~50 kDa band corresponding to IRF8 was observed in THP-1, Raji, Ramos as compared to K-562, T98-G which are reported to be low for IRF8 expression. Whole cell extracts (40 µg lysate) of THP-1 (Lane 1), Raji (Lane 2), Ramos (Lane 3), K-562 (Lane 4) and T98-G (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocell... View More
Product Details
740242MP647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
GW4CML3
Conjugate
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H-ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8; unnamed protein product
Gene Aliases: H-ICSBP; ICSBP; ICSBP1; IMD32A; IMD32B; IRF-8; IRF8
UniProt ID: (Human) Q02556
Entrez Gene ID: (Human) 3394
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
plasmacytoid dendritic cell differentiation
germinal center B cell differentiation
follicular B cell differentiation
immune system process
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
phagocytosis
autophagy
immune response
response to bacterium
myeloid cell differentiation
regulation of type I interferon production
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
defense response to bacterium
defense response to protozoan
positive regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to lipopolysaccharide
cellular response to interferon-gamma
dendritic cell differentiation
defense response to other organism
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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