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          • Primary Antibodies ›
          • BCL-2 Antibodies

          Invitrogen

          Bcl-2 Monoclonal Antibody (Bcl-2-100), Alexa Fluor™ Plus 594

          View all (106) BCL-2 antibodies

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          Cite Bcl-2 Monoclonal Antibody (Bcl-2-100), Alexa Fluor™ Plus 594

          Additional Information:
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          • Antibody Testing Data (4)
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

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          Bcl-2 Antibody (755-8800-94) in IHC (P)

          Immunohistochemical analysis of Bcl-2 was performed on FFPE human Tonsil tissue. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 2 (pH 9) for 20-mins, followed by a 5-min cool down, a 1-min wash with ddH2O, and then transferred to 1X PBS. Tissues were then blocked with 3% BSA/5% normal goat serum in 1X PBS for 1-hr at RT. Following blocking ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Bcl-2 Monoclonal Antibody (Bcl-2-100), Alexa Fluor™ Plus 594

          Product Details

          755-8800-94

          Applications
          Tested Dilution
          Publications

          Multiplex Immunohistochemistry (mIHC)

          8 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          8 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          Bcl-2-100

          Immunogen

          Synthetic peptide corresponding to residues 41-54 of the human Bcl-2 protein. This sequence is not shared by the mouse Bcl-2 protein.
          3D Epitope / Immunogen

          Conjugate

          Alexa Fluor™ Plus 594 Alexa Fluor™ Plus 594 Alexa Fluor™ Plus 594
          • Alexa Fluor Plus 647 
          • Request custom conjugation

          Excitation/Emission Max

          590/618 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer

          Contains

          0.05% sodium azide

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Wet ice

          Product Specific Information

          Description: This Bcl-2-100 monoclonal antibody reacts to human Bcl-2 (B-cell Lymphoma 2), the founding member of a family of pro-apoptotic proteins which includes Bcl-XL, Bcl-w, and Mcl-1. Bcl-2 plays a role in protecting against apoptosis-inducing insults such as dexamethasone and staurosporine, as well as irradiation and cytokine withdrawal. The mechanism is thought to include regulation of mitochondrial membrane permeability, resulting in sequestration of caspase proforms, or by preventing the release of apoptosis-mediating mitochondrial factors, such as cytochrome c and AIF (apoptosis-inducing factor), into the cytoplasm. Bcl-2 is localized to the cytoplasmic side of the mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. Interestingly Bcl-2 does not play a role in preventing cell death of autoreactive T cells in the thymus or Fas-mediated apoptosis. Two isoforms, termed alpha and beta of Bcl-2 have been identified and are a result of alternative splicing at the C-terminus.

          Applications Reported: This Bcl-2-100 antibody has been reported for use in immunoblotting (WB), immunohistochemistry, immunocytochemistry and flow cytometry.

          Applications Tested: This Bcl-2-100 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 8 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

          Target Information

          BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl 2; bcl-2 protein; bcl2-alpha protein; bcl2-beta protein; bcl2-Ig fusion gene; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50

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          Gene Aliases: Bcl-2; BCL2; PPP1R50

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          UniProt ID: (Human) P10415

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          Entrez Gene ID: (Human) 596

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          Function(s)
          protease binding protein binding channel activity channel inhibitor activity protein phosphatase binding ubiquitin protein ligase binding identical protein binding sequence-specific DNA binding protein heterodimerization activity BH3 domain binding protein phosphatase 2A binding binding, bridging DNA-binding transcription factor binding
          Process(es)
          G1/S transition of mitotic cell cycle protein polyubiquitination mitotic cell cycle cell morphogenesis ossification ovarian follicle development metanephros development ureteric bud development branching involved in ureteric bud morphogenesis behavioral fear response leukocyte homeostasis B cell homeostasis B cell apoptotic process kidney development release of cytochrome c from mitochondria regulation of cell-matrix adhesion lymphocyte homeostasis lymphoid progenitor cell differentiation B cell lineage commitment T cell lineage commitment immune system development negative regulation of B cell apoptotic process response to ischemia renal system process melanin metabolic process regulation of nitrogen utilization cellular calcium ion homeostasis autophagy apoptotic process humoral immune response cellular response to DNA damage stimulus response to oxidative stress actin filament organization axonogenesis female pregnancy cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation male gonad development extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway in response to oxidative stress apoptotic mitochondrial changes response to radiation response to xenobiotic stimulus response to toxic substance post-embryonic development organ morphogenesis response to iron ion response to UV-B response to gamma radiation regulation of gene expression regulation of autophagy negative regulation of autophagy negative regulation of calcium ion transport into cytosol regulation of glycoprotein biosynthetic process mesenchymal cell development positive regulation of neuron maturation smooth muscle cell migration positive regulation of smooth muscle cell migration cell migration cochlear nucleus development gland morphogenesis hemopoiesis B cell differentiation T cell differentiation negative regulation of ossification positive regulation of cell growth negative regulation of cell growth melanocyte differentiation negative regulation of cell migration positive regulation of B cell proliferation hair follicle morphogenesis axon regeneration regulation of protein stability endoplasmic reticulum calcium ion homeostasis glomerulus development negative regulation of cellular pH reduction regulation of protein localization myeloid cell apoptotic process negative regulation of myeloid cell apoptotic process T cell differentiation in thymus osteoblast proliferation negative regulation of osteoblast proliferation response to cytokine response to nicotine organ growth regulation of growth positive regulation of multicellular organism growth B cell proliferation cellular response to glucose starvation response to hydrogen peroxide neuron maturation regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process regulation of programmed cell death CD8-positive, alpha-beta T cell lineage commitment pigmentation negative regulation of neuron apoptotic process ear development regulation of viral genome replication positive regulation of melanocyte differentiation negative regulation of mitotic cell cycle retinal cell programmed cell death negative regulation of retinal cell programmed cell death regulation of mitochondrial membrane permeability focal adhesion assembly developmental pigmentation regulation of developmental pigmentation positive regulation of developmental pigmentation spleen development thymus development response to steroid hormone digestive tract morphogenesis developmental growth oocyte development skeletal muscle fiber development positive regulation of skeletal muscle fiber development pigment granule organization stem cell development homeostasis of number of cells within a tissue B cell receptor signaling pathway response to glucocorticoid neuron apoptotic process defense response to virus establishment of localization in cell regulation of cell cycle regulation of mitochondrial membrane potential negative regulation of mitochondrial depolarization regulation of calcium ion transport hematopoietic stem cell differentiation calcium ion transport into cytosol intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress lymphocyte apoptotic process negative regulation of lymphocyte apoptotic process T cell apoptotic process negative regulation of T cell apoptotic process cellular response to hypoxia reactive oxygen species metabolic process dendritic cell apoptotic process motor neuron apoptotic process extrinsic apoptotic signaling pathway in absence of ligand cell-cell adhesion negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of intracellular signal transduction epithelial cell apoptotic process negative regulation of epithelial cell apoptotic process negative regulation of G1/S transition of mitotic cell cycle negative regulation of dendritic cell apoptotic process negative regulation of motor neuron apoptotic process negative regulation of anoikis regulation of apoptotic signaling pathway negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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