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Invitrogen

beta Catenin Monoclonal Antibody (15B8), Alexa Fluor™ Plus 647

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Product Details

756-2567-94

Applications
Tested Dilution
Publications

Multiplex Immunohistochemistry (mIHC)

2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

15B8

Conjugate

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

Excitation/Emission Max

658/675 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer

Contains

0.05% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Wet ice

Product Specific Information

Description: The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt and beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.

Applications Reported: This 15B8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunoprecipitation, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. (Fluorochrome conjugated 15B8 is recommended for use in intracellular flow cytometry).

Applications Tested: This 15BB antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Target Information

Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bcatenin; beta 1 88kDa; Betacatenin; C20orf33; Cadherin associated protein; catenin; catenin (cadherin-associated protein), beta 1, 88kDa; Catenin b 1; Catenin b1; Catenin beta1; Catenin β 1; Catenin β1; CTNB1; dJ633O20.1; DKFZp686D02253; FLJ25606; FLJ37923; NAP; NYD-SP19; OTTHUMP00000209289; P14L; PP8304; RP5-1118M15.1; unnamed protein product; β catenin; βcatenin

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Gene Aliases: armadillo; CTNNB; EVR7; MRD19; NEDSDV

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UniProt ID: (Human) P35222

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Entrez Gene ID: (Human) 1499

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Function(s)
transcription cofactor binding transcription corepressor binding chromatin binding transcription coactivator activity receptor binding protein binding ligand-dependent nuclear receptor binding enzyme binding kinase binding protein kinase binding phosphatase binding protein phosphatase binding estrogen receptor binding ubiquitin protein ligase binding ion channel binding alpha-catenin binding cadherin binding SMAD binding RNA polymerase II sequence-specific DNA binding transcription factor binding I-SMAD binding disordered domain specific binding DNA-binding transcription factor binding histone methyltransferase binding
Process(es)
protein polyubiquitination osteoblast differentiation epithelial to mesenchymal transition positive regulation of neuroblast proliferation outflow tract morphogenesis cell adhesion epidermal growth factor receptor signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation response to xenobiotic stimulus negative regulation of gene expression regulation of epithelial to mesenchymal transition positive regulation of epithelial to mesenchymal transition positive regulation of heparan sulfate proteoglycan biosynthetic process negative regulation of angiogenesis stem cell population maintenance hypothalamus development neuron differentiation midbrain development hindbrain development regulation of centriole-centriole cohesion regulation of protein ubiquitination positive regulation of telomere maintenance via telomerase response to estradiol positive regulation of transcription elongation from RNA polymerase II promoter negative regulation of protein sumoylation positive regulation of homotypic cell-cell adhesion adherens junction assembly protein localization to cell surface hair cell differentiation detection of muscle stretch embryonic skeletal limb joint morphogenesis astrocyte-dopaminergic neuron signaling positive regulation of apoptotic process negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process cell-cell adhesion mediated by cadherin positive regulation of cell differentiation regulation of angiogenesis positive regulation of angiogenesis negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of mitotic cell cycle, embryonic regulation of fibroblast proliferation embryonic organ development positive regulation of skeletal muscle tissue development regulation of smooth muscle cell proliferation regulation of neurogenesis negative regulation of neurogenesis detection of mechanical stimulus regulation of timing of anagen regulation of synapse assembly canonical Wnt signaling pathway mesenchymal to epithelial transition long term synaptic depression endothelial tube morphogenesis sympathetic ganglion development cranial ganglion development regulation of centromeric sister chromatid cohesion cellular response to growth factor stimulus cellular response to indole-3-methanol regulation of nephron tubule epithelial cell differentiation mesenchymal stem cell differentiation negative regulation of canonical Wnt signaling pathway regulation of calcium ion import synaptic vesicle clustering cell-cell adhesion positive regulation of odontoblast differentiation negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway cranial skeletal system development midbrain dopaminergic neuron differentiation positive regulation of blood vessel branching neuron projection extension embryonic brain development dorsal root ganglion development regulation of protein localization to cell surface positive regulation of neural precursor cell proliferation
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