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Invitrogen
ABI1 Polyclonal Antibody
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FIGURE: 1 / 7
ABI1 Antibody (PA5-78705) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of ABI1 in U2OS cells using ABI1 Polyclonal Antibody (Product # PA5-78705). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. Cy3 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropri... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78705
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ABI1 (483-494aa WYEGVCNRVTGL).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, human MCF-7 whole cell, human U87 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell, mouse C2C12 whole cell. IHC: human lung cancer tissue, human colorectal adenocarcinoma tissue. ICC/IF: U2OS cell. Flow: MCF-7 cell.
Target Information
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Abelson interactor 1; abi-1; Abi-1 long; ABI1; abl-binding protein 4; Abl-interactor protein 1 long; ablphilin-1; adapter; binds to abelson kinase; EL2; eps8 binding protein; eps8 binding protein (e3B1), alternatively spliced; eps8 SH3 domain-binding protein; eps8-binding protein; HPP; HS3; interactor protein AblBP4; nap1 binding protein; skeletin; HSSH3BP1; spectrin SH3 domain binding protein 1; spectrin SH3 domain-binding protein 1; SPH3; SPTA; unnamed protein product
Gene Aliases: ABI-1; ABLBP4; E3B1; NAP1; NAP1BP; SSH3BP; SSH3BP1
UniProt ID: (Human) Q8IZP0, (Rat) Q9QZM5, (Mouse) Q8CBW3
Entrez Gene ID: (Human) 10006, (Rat) 79249, (Mouse) 11308
somitogenesis
neuron migration
megakaryocyte development
dendrite morphogenesis
cell projection morphogenesis
positive regulation of protein tyrosine kinase activity
lamellipodium morphogenesis
postsynapse to nucleus signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
actin polymerization or depolymerization
negative regulation of cell proliferation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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