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Bethyl Laboratories
AIF Polyclonal Antibody
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Product Details
A302-782A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 100 and 150 of human Apoptosis-Inducing Factor, Mitochondrion-Associated, 1.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AG1; AGR1; apoptogenic protein; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; auditory neuropathy, X-linked recessive 1; cell death factor; ER protein 18; ER protein 19; ERP18; ERP19; hAG-1; hTLP19; MGC111425; NADH oxidase; pcd8; PDCD8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; unnamed protein product; UNQ713/PRO1376
Gene Aliases: AIF; AIFM1; AUNX1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8; SEMDHL
UniProt ID: (Human) O95831
Entrez Gene ID: (Human) 9131
response to ischemia
apoptotic process
response to toxic substance
neuron differentiation
mitochondrial respiratory chain complex assembly
positive regulation of apoptotic process
positive regulation of programmed cell death
positive regulation of neuron apoptotic process
protein import into mitochondrial intermembrane space
positive regulation of necroptotic process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hydrogen peroxide
cellular response to estradiol stimulus
cellular response to hypoxia
cellular response to nitric oxide
mitochondrial disulfide relay system
response to L-glutamate
cellular response to aldosterone
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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