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          • Primary Antibodies ›
          • AKT Pan Antibodies

          Rockland

          AKT Polyclonal Antibody

          View all (33) AKT Pan antibodies

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          Cite AKT Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          AKT Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          AKT Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          AKT Antibody (200-401-N98) in WB

          Western Blot of Anti-AKT (RABBIT) Antibody. Lane 1: SuperSignal MW markers. Lane 2: rAKT1. Lane 3: rAKT2. Lane 4: rAKT3. Load: 50 ng per lane. Primary antibody: Anti- AKT (RABBIT) Antibody at 1:1,000 for 2 hours at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:20,000 for 1 hour at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070), 1/2 hour at RT. Predicted/Observed size: 56kDa, 56kDa for AKT1, 2, and 3. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          AKT Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          200-401-N98

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          -

          Immunohistochemistry (IHC)

          1:500-1:2,000
          -

          ELISA (ELISA)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          1:1,000-1:5,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          0.02M potassium phosphate, pH 7.2, with 0.5M NaCl

          Contains

          0.01% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

          AKT Antibody reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.

          Target Information

          AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: AKT1 kinase; Akt1m protein; AKT2 kinase; AKT3 kinase; DKFZp434N0250; kinase Akt1; MGC99656; murine thymoma viral (v-akt) oncogene homolog 1; murine thymoma viral (v-akt) oncogene homolog 2; pan akt; PKB; PKB alpha; PKB beta; PKB gamma; Protein kinase Akt-2; Protein kinase Akt-3; Protein kinase B; Protein kinase B alpha; Protein kinase B beta; Protein kinase B gamma; protein kinase B, beta; protein kinase B, gamma; protein kinase B-alpha; Proto-oncogene c-Akt; RAC protein kinase alpha RAC-PK alpha; RAC protein kinase beta; RAC protein kinase beta RAC-PK beta; RAC-alpha serine/threonine-protein kinase; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; STK-2; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; thymoma viral proto-oncogene 2; thymoma viral proto-oncogene 3; v-akt murine thymoma viral oncogene 3; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)

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          Gene Aliases: 2410016A19Rik; AI851531; Akt; AKT1; AKT2; AKT3; AW554154; D930002M15Rik; Nmf350; PKB; PKB/Akt; PKBalpha; PKBbeta; PKBG; RAC

          View more View less

          UniProt ID: (Mouse) P31750, (Rat) P47196, (Rat) P47197, (Mouse) Q60823, (Rat) Q63484, (Mouse) Q9WUA6

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          Entrez Gene ID: (Mouse) 11651, (Rat) 24185, (Rat) 25233, (Mouse) 11652, (Rat) 29414, (Mouse) 23797

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          Function(s)
          nucleotide binding protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups enzyme binding protein kinase binding nitric-oxide synthase regulator activity identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding non-receptor serine/threonine protein kinase protein modifying enzyme
          Process(es)
          protein import into nucleus, translocation osteoblast differentiation maternal placenta development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation carbohydrate metabolic process glycogen metabolic process glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process translation regulation of translation protein phosphorylation negative regulation of protein kinase activity transport apoptotic process activation-induced cell death of T cells inflammatory response cytoskeleton organization signal transduction multicellular organism development germ cell development nervous system development aging insulin receptor signaling pathway apoptotic mitochondrial changes carbohydrate transport response to hormone negative regulation of autophagy negative regulation of plasma membrane long-chain fatty acid transport positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process negative regulation of endopeptidase activity regulation of neuron projection development glucose transport phosphorylation protein ubiquitination peptidyl-serine phosphorylation cell projection organization protein catabolic process positive regulation of cell growth regulation of cell migration regulation of myelination positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle lipopolysaccharide-mediated signaling pathway negative regulation of fatty acid beta-oxidation response to food positive regulation of cellular protein metabolic process peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus regulation of protein localization positive regulation of peptidyl-serine phosphorylation response to fluid shear stress intracellular signal transduction cellular response to vascular endothelial growth factor stimulus cellular response to decreased oxygen levels glucose homeostasis anagen positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import negative regulation of JNK cascade positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to organic cyclic compound cellular response to hypoxia positive regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis cellular response to peptide response to oxidative stress hyaluronan metabolic process response to growth hormone response to insulin-like growth factor stimulus response to osmotic stress positive regulation of signal transduction positive regulation of gene expression response to muscle activity positive regulation of cell migration positive regulation of vesicle fusion response to nutrient levels positive regulation of fatty acid beta-oxidation response to insulin cellular response to hormone stimulus negative regulation of RNA splicing positive regulation of positive chemotaxis intracellular protein transmembrane transport protein localization to plasma membrane positive regulation of protein targeting to membrane positive regulation of cell motility positive regulation of glucose import in response to insulin stimulus activation of GTPase activity mitochondrial genome maintenance peptidyl-threonine phosphorylation
          It has to be done as per old AB suggested Products section.

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