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Rockland
AMBRA1 Polyclonal Antibody
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AMBRA1 Antibody (600-401-Y08) in ICC/IF
Immunofluorescence Microscopy of Ambra1 antibody. Tissue: Human brain cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Ambra1 antibody at 20 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Ambra1 as a red fluorescent signal.
产品信息
600-401-Y08
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-Ambra1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the C-terminus of human Ambra1.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: activating molecule in beclin-1-regulated autophagy; activating molecule in Beclin1-regulated autophagy; Activating molecule in BECN1-regulated autophagy protein 1; autophagy/beclin-1 regulator 1; DDB1 and CUL4 associated factor 3; DDB1- and CUL4-associated factor 3; ischemia related factor NYW-1; WD repeat domain 94
基因别名: 2310079H06Rik; A130023A14; AA474864; AMBRA1; AV021921; D030051N19Rik; DCAF3; KIAA1736; mKIAA1736; Nyw1; WDR94
UniProt ID: (Human) Q9C0C7, (Mouse) A2AH22
Entrez Gene ID: (Human) 55626, (Rat) 59319, (Mouse) 228361
autophagy
negative regulation of cell proliferation
neural tube development
negative regulation of neuron apoptotic process
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of mitophagy
regulation of autophagosome assembly
macroautophagy
cell differentiation
parkin-mediated mitophagy in response to mitochondrial depolarization
mitophagy in response to mitochondrial depolarization
multicellular organism development
nervous system development
embryo development
