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Invitrogen
AMID Recombinant Rabbit Monoclonal Antibody (AMID-Clone 46)
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FIGURE: 1 / 4
AMID Antibody (705336R) in WB
Western blot was performed using AMID Recombinant Rabbit Monoclonal Antibody (AMID-Clone 46) (Product # 705336R) and a ~40 kDa band corresponding to AMID was observed in Hep G2 and KATO III cell lines which are reported to be high expressors for AMID as compared to Huh-7, HCT 116, HeLa and K-562 cell lines. Whole cell extracts (30 μg lysate) of Hep G2 (Lane 1), KATO III (Lane 2), Huh-7 (Lane 3), HCT 116 (Lane 4), HeLa (Lane 5) and K-562 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved p... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
705336R
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
AMID-Clone 46
Immunogen
Protein corresponding to human AMID [aa1-aa373]
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Recombinant Rabbit monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
The p53 tumor suppressor protein induces cell cycle arrest or apoptosis in response to cellular stresses. PRG3 (p53-responsive gene 3), is induced specifically under p53-dependent apoptotic conditions in human colon cancer cells. PRG3 has significant homology to bacterial oxidoreductases and the apoptosis-inducing factor, AIF. Unlike AIF, PRG3 localizes in the cytoplasm and its ectopic expression induces apoptosis. The PRG3 gene is thus a novel p53 target gene in a p53-dependent apoptosis pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AIF family member 2; apoptosis inducing factor mitochondria associated 2; apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death; apoptosis-inducing factor 2; apoptosis-inducing factor, mitochondrion-associated, 2; apoptosis-inducing factor-like flavoprotein; ferroptosis suppressor 1; FSP-1; MBP2; MBPH; MGC126662; MGC141971; p53-responsive gene 3 protein; PRG3; p53-inducible; apoptotic protein; RP11-367H5.2; unnamed protein product
Gene Aliases: AMID; FSP1; PRG3
UniProt ID: (Human) Q9BRQ8
Entrez Gene ID: (Human) 84883
DNA binding
electron-transferring-flavoprotein dehydrogenase activity
protein binding
NADPH dehydrogenase (quinone) activity
oxidoreductase activity
oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
NADH dehydrogenase (quinone) activity
flavin adenine dinucleotide binding
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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