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Invitrogen
AMOTL2 Polyclonal Antibody
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FIGURE: 1 / 9
AMOTL2 Antibody (PA5-78770) in WB
Western blot analysis of AMOTL2 in Lane 1: human placenta tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with AMOTL2 polyclonal antibody (Product # PA5-78770) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP seconda... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78770
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human AMOTL2 recombinant protein (Position: A401-Q480).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL once reconstituted
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, rat brain tissue, rat lung tissue, mouse lung tissue, mouse liver tissue.
Target Information
Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: LCCP; Leman coiled-coil protein; Leman coiled-coil protein; unnamed protein product
Gene Aliases: LCCP; MASCOT; mKIAA0989
UniProt ID: (Human) Q9Y2J4, (Rat) G3V735, (Mouse) Q8K371
Entrez Gene ID: (Human) 51421, (Rat) 65157, (Mouse) 56332
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
endothelial cell morphogenesis
establishment of cell polarity involved in ameboidal cell migration
actin cytoskeleton organization
regulation of cell migration
hippo signaling
positive regulation of cellular protein localization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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