Invitrogen

ANO6 Polyclonal Antibody

2个已发表图片

3 篇参考文献

查看其他11个ANO6抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (4)
已发表图片 (2)

ANO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ANO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ANO6 Antibody (PA5-58610) in IHC (P)

Immunohistochemical analysis of ANO6 in human testis using ANO6 Polyclonal Antibody (Product # PA5-58610) shows moderate membranous positivity in cells in seminiferous ducts.

ANO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ANO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ANO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ANO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ANO6 Antibody in Western Blot (WB)

ANO6 Antibody in Western Blot (WB)

Product Image Gallery

ANO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ANO6 in human testis using ANO6 Polyclonal Antibody (Product # PA5-58610) shows moderate membranous positivity in cells in seminiferous ducts.

ANO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ANO6 in human placenta using ANO6 Polyclonal Antibody (Product # PA5-58610) shows moderate membranous positivity in trophoblastic cells.

ANO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ANO6 in human kidney using ANO6 Polyclonal Antibody (Product # PA5-58610) shows strong membranous positivity in cells in glomeruli.

ANO6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ANO6 in human liver using ANO6 Polyclonal Antibody (Product # PA5-58610) shows moderate membranous positivity in hepatocytes.

ANO6 Antibody in Western Blot (WB) — Figure 5 B1 signaling activity is reduced in cells lacking ANO6. A , confirmation of ANO6 KO in HEK293T cell line: on left , Western blot of wildtype HEK293T lysates versus lysates from ANO6 KO cell line immunoblotted for ANO6 and beta-tubulin. B , flow histogram of mock-transfected ( gray ) wildtype HEK293T cells (+annexin V) overlaid with mock-transfected ANO6 KO cells ( blue ; +annexin V) demonstrates that ANO6 KO cells exhibit lower baseline PS exposure than do wildtype HEK293T cells. Lower probe-positive cells were observed in the ANO6 KO cells. C , quantification of PS exposure in ANO6 KO mutant cell line compared with WT HEK293T cells: Mean fluorescence intensity (MFI) shown with ANO6 KO condition normalized to mock-transfected condition. PS exposure was reduced by 26% in the ANO6 KO cells (mean +- SEM is shown, unpaired t test, p = 0.0004, n = 5). D , B1FL activation of SRF-luciferase in ANO6 KO cells was reduced by 67% relative to matched wildtype HEK293T cells (normalized to cell line, mean +- SEM is shown, ordinary one-way ANOVA with Tukey's multiple comparisons test, p < 0.0001, n = 6 for HEK293T condition, n = 5 for ANO6 KO condition, ROUT method used at 10% to remove one outlier from both conditions). ANO6, anoctamin 6; B1FL, full-length B1; HEK293T, human embryonic kidney 293T cell line; PS, phosphatidylserine.

ANO6 Antibody in Western Blot (WB) — Figure 5. Ano5 -/- muscle cells exhibit perturbed Ca 2+ -PLS and PLS-associated ionic current. (a) Simultaneous whole-cell patch clamp and annexin V-Alexa Fluor 568 binding of primary MPCs differentiated for ~24 hr. Images on the left show bright-field micrographs of patch-clamped MPCs. Center and right images show annexin V-Alexa Fluor 568 binding. Scale bars, 10 mum. Below the images are ionic currents recorded using voltage steps between +100 and -100 mV in 20-mV increments from a holding potential of 0 mV. (b) Quantification of Ca 2+ -PLS in MPCs (left), and average current of differentiated myoblasts at +100 mV (right). The statistical difference in Ca 2+ -PLS between genotypes was calculated using a Fisher exact test (***, P = 0.0001). The current at 20 min was statistically evaluated using a paired Student's t test (*, P = 0.026). The white numbers in the columns indicate the number of cells assayed. The same cells were measured at 3 and 20 min. Error bars indicate SEM. (c) Western blot of native ANO6 levels in wild-type and Ano5 knockout MPCs differentiated for ~24 h.

Immunogen image

ANO6 Polyclonal Antibody

Immunogen Image ANO6 Polyclonal Antibody

产品信息

PA5-58610

应用

建议稀释比

已发表文章

免疫印迹 (WB)

-

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免疫组化（石蜡） (IHC (P))

1:500-1:1,000

-

免疫沉淀 (IP)

-

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human ANO6. Recombinant protein control fragment (Product #RP-97863).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Immunogen sequence: EEDDDDGDIV LENLGQTIVP DLGSLESQHD FRTPEFEEFN GKPDSLFFND GQRRIDFVLV YEDESRKETN KKGTNEKQRR KRQAYESNLI CHGLQLEATR SVLD

Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 79%.

靶标信息

May act as a calcium-activated chloride channel.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Anoctamin-6; SCAN channel; Small-conductance calcium-activated nonselective cation channel; Transmembrane protein 16F

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基因别名: ANO6; BDPLT7; SCTS; TMEM16F

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UniProt ID: (Human) Q4KMQ2

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Entrez Gene ID: (Human) 196527

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calcium activated cation channel activity intracellular calcium activated chloride channel activity voltage-gated ion channel activity voltage-gated chloride channel activity protein binding phospholipid scramblase activity protein homodimerization activity

dendritic cell chemotaxis activation of blood coagulation via clotting cascade cation transport chloride transport lipid transport blood coagulation phospholipid scrambling positive regulation of bone mineralization bleb assembly ion transmembrane transport positive regulation of ion transmembrane transport purinergic nucleotide receptor signaling pathway bone mineralization involved in bone maturation sodium ion transmembrane transport positive regulation of apoptotic process negative regulation of cell volume pore complex assembly positive regulation of phagocytosis, engulfment calcium activated phosphatidylserine scrambling calcium activated phosphatidylcholine scrambling calcium activated galactosylceramide scrambling calcium ion transmembrane transport positive regulation of monocyte chemotaxis phosphatidylserine exposure on blood platelet positive regulation of potassium ion export chloride transmembrane transport regulation of anion transmembrane transport positive regulation of endothelial cell apoptotic process