Invitrogen

AP2M1 Polyclonal Antibody

Name: AP2M1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-20745

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2个已发表图片

1 篇参考文献

查看其他20个AP2M1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

AP2M1 Antibody in Immunocytochemistry (ICC/IF)

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AP2M1 Antibody (PA5-20745) in ICC/IF

Immunofluorescence of AP2M1 in Mouse Kidney cells with AP2M1 Polyclonal Antibody (Product # PA5-20745) at 20 µg/mL.

AP2M1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

AP2M1 Antibody — Figure 5 PSD-95 enhances the binding and tyrosine phosphorylation of DHHC5 by Fyn kinase. ( a - d ) Fourteen DIV hippocampal neurons were stimulated, lysed at the indicated time points, immunoprecipitated with anti- ( a ) DHHC5 or ( c ) Fyn and blots probed with the indicated antibodies. ( a , b ) DHHC5 tyrosine phosphorylation (phY; P =0.007, F 6,14 =7.749) and DHHC5/Fyn interactions ( P <0.001, F 6,14 =11.06) are decreased transiently, whereas DHHC5/AP2mu interactions are increased transiently following cLTP ( P =0.0076, F 6,14 =7.78). ( c , d ) Non-phosphoryated tyrosine 420 Fyn levels ( P =0.0001, F 6,14 =11.24) and STEP61/Fyn interactions ( P =0.004, F 6,14 =5.549) are transiently increased following cLTP. ( e - i ) HEK293T cells were transfected with the indicated HA-DHHC5, PSD-95-GFP or Fyn constructs for 36 h. ( e , f ) PSD-95 enhances Fyn/DHHC5 WT but not Fyn/DHHC5 DeltaPDZb interactions ( P =0.0014, F 2,6 =24.11). ( e , g ) PSD-95 enhances Fyn-mediated phosphorylation of DHHC5 WT but not DHHC5 DeltaPDZb ( P =0.0104, F 2,6 =10.75). ( h , i ) Lysates were biotinylated and immunoprecipitated with neutravidin-coated beads and blots probed with the indicated antibodies. ( i ) Normalized intensity of DHHC5 in the surface fraction ( P <0.001, F 7,16 =43.23). ( b , d , f , g , i ) n =3 blots from 3 separate cultures. All graphs display mean+-s.e.m. */ # P <0.05, **/ ## P <0.01, *** P <0.001; one-way analysis of variance; Tukey's post-hoc test. Asterisks and cross-hatches ab {TM}

Immunogen image

Immunogen Image AP2M1 Polyclonal Antibody

产品信息

PA5-20745

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化 (IHC)

2.5 µg/mL

免疫细胞化学 (ICC/IF)

20 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Chicken / IgY

分类

Polyclonal

类型

Antibody

抗原

A 16 amino acid peptide near the carboxy terminus of human AP2M1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11154294

产品详细信息

A suggested positive control is human kidney tissue lysate.

PA5-20745 can be used with blocking peptide PEP-0859.

靶标信息

The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: adapter-related protein complex 2 mu subunit; adapter-related protein complex 2 subunit mu; Adaptin-mu2; Adaptor protein complex AP-2 subunit mu; adaptor related protein complex 2, mu 1 subunit; Adaptor-related protein complex 2 subunit mu; adaptor-related protein complex 2, mu 1 subunit; AP-2 complex subunit mu; AP-2 mu 2 chain; AP-2 mu chain; clathrin adaptor complex AP2, mu subunit; clathrin assembly protein complex 2 medium chain; Clathrin assembly protein complex 2 mu medium chain; clathrin coat adaptor protein AP50; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; clathrin-associated/assembly/adaptor protein, medium 1; coat assembly protein complex 50 kD; HA2 50 kDA subunit; Mu2-adaptin; Plasma membrane adaptor AP-2 50 kDa protein; plasma membrane adaptor AP-2 50kDA protein

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基因别名: AP2M1; AP50; CLAPM1; KIAA0109; mu2

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UniProt ID: (Human) Q96CW1, (Rat) P84092, (Mouse) P84091

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Entrez Gene ID: (Human) 1173, (Rat) 116563, (Mouse) 11773

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功能

signal sequence binding transporter activity protein binding lipid binding clathrin adaptor activity ion channel binding low-density lipoprotein particle receptor binding membrane traffic protein

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