Invitrogen

ATP5A1 Monoclonal Antibody (7H10BD4F9)

Name: ATP5A1 Monoclonal Antibody (7H10BD4F9)
Brand: Invitrogen
SKU: 459240

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51 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

ATP5A1 Antibody in Immunocytochemistry (ICC/IF)

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ATP5A1 Antibody (459240) in ICC/IF

Bovine pulmonary artery endothelial cells: Mitochondria were labeled using an anti-OxPhos Complex V subunit a, mouse IgG_2b monoclonal antibody (Product # 459240) prelabeled with the Zenon® Alexa Fluor® 555 Mouse IgG_2b Labeling Kit (Product # Z-25205). Tubulin was detected with an anti-a-tubulin mouse IgG_2b monoclonal antibody prelabeled with the Zenon® Alexa Fluor® 488 Mouse IgG_2b Labeling Kit (Product # Z-25202), and The nucleus was stained with DAPI (Product # D1306, D3571, D21490).

ATP5A1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

ATP5A1 Antibody in Immunohistochemistry (IHC)

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产品信息

459240

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

5 µg/mL

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流式细胞分析 (Flow)

1 µg/mL

免疫沉淀 (IP)

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功能检测 (Functional)

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亲和纯化 (AP)

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其他PubMed (Misc)

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产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

Bovine, C. elegans, Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

7H10BD4F9

抗原

Bovine Complex V

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

IgG fraction

保存液

HEPES buffered saline

内含物

0.02% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2532234

产品详细信息

Use Isolated mitochondria from Human, Rat, Bovine and Mouse Heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HL-60 cells for positive controls.

靶标信息

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: alpha subunit ATP synthase isoform precursor (EC 3.6.1.34); ATP synthase alpha chain, mitochondrial; ATP synthase alpha subunit; ATP synthase alpha subunit precursor; ATP synthase F1 subunit alpha; ATP synthase subunit alpha, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2; ATP sythase (F1-ATPase) alpha subunit; epididymis secretory sperm binding protein Li 123m; iso; mitochondrial ATP synthetase, oligomycin-resistant; mitochondrial H+-ATP synthase alpha subunit; modifier of Min 2

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基因别名: AI035633; AL022851; AL023067; ATP5A; ATP5A1; ATP5A2; ATP5AL2; ATP5F1A; ATPM; COXPD22; D18Ertd206e; hATP1; HEL-S-123m; MC5DN4; MOM2; OMR; ORM