Invitrogen

Actin Monoclonal Antibody (ACTN05 (C4))

Name: Actin Monoclonal Antibody (ACTN05 (C4))
Brand: Invitrogen
SKU: MA5-11869

59个已发表图片

393 篇参考文献

查看其他15个Actin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (11)
已发表图片 (59)

Actin Antibody in Immunocytochemistry (ICC/IF)

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Actin Antibody (MA5-11869) in ICC/IF

Immunofluorescent analysis of Actin pan (green) showing staining in the cytoplasm of T-47D cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Actin pan monoclonal antibody (Product # MA5-11869) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLi... View More

Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Actin Antibody in Immunoprecipitation (IP)

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Immunogen image

Immunogen Image Actin Monoclonal Antibody (ACTN05 (C4))

产品信息

MA5-11869

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5 µg/mL

查看352篇已发表文章 352篇已发表文章

免疫组化 (IHC)

查看6篇已发表文章 6篇已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:400

免疫细胞化学 (ICC/IF)

1:10-1:200

查看6篇已发表文章 6篇已发表文章

免疫沉淀 (IP)

2 µg

查看6篇已发表文章 6篇已发表文章

免疫电镜技术 (IM)

查看1篇已发表文章 1篇已发表文章

亲和纯化 (AP)

查看18篇已发表文章 18篇已发表文章

其他PubMed (Misc)

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Bovine, Dog, Chicken, Human, Mouse, Pig, Protozoa, Rabbit, Rat

已发表种属

C. elegans, Chicken, Chordate, Dog, Ferret, Fish, Fruit fly, Hamster, Human, Insect, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Tag, Xenopus, Yeast, Zebrafish

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

ACTN05 (C4)

抗原

Chicken gizzard actin

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

15mM sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11004139

产品详细信息

MA5-11869 reacts with all six known vertebrate isoforms of actin (MW ~42kDa), and with two cytoplasmic actins (beta and gamma), which are highly homologous to each other but differ from the muscle actins by ~25 amino acid residues. This product is highly recommended for monitoring total protein load on Western blots.

Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Suggested positive controls are HeLa cells and skeletal muscle.

MA5-11869 is a suggested replacement to Product # 82353, clone # C4.

靶标信息

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: a-actin; ACTB; actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 2; Actin, cytoplasmic type 5; actin, cytoplasmic, type 5; actin, gamma 1 propeptide; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin 1; alpha actin 2; Alpha-actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; Atcb; B-actin; Bact; actin; beta actin; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; cardiac actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoplasmic 1; cytoplasmic 2; cytoplasmic beta-actin; cytoplasmic type 5; cytoskeletal beta actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; gamma 1 propeptide; gamma non-muscle actin; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; skeletal alpha actin; smooth muscle alpha-actin; Smooth muscle gamma-actin; type 5; unnamed protein product; vascular smooth muscle alpha-actin

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基因别名: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; ACT-4; ACT-5; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; ASD5; ASMA; Bact; beta-actin; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E430023M04Rik; E51; GAMMA-ACTIN; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMGA; VSCM

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功能

protein binding ATP binding structural constituent of cytoskeleton myosin binding ADP binding protein kinase binding kinesin binding Tat protein binding identical protein binding nitric-oxide synthase binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding nucleosomal DNA binding structural constituent of postsynaptic actin cytoskeleton ATPase activity ubiquitin protein ligase binding profilin binding nucleotide binding molecular_function actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

参与通路

skeletal muscle thin filament assembly skeletal muscle fiber development muscle contraction response to mechanical stimulus response to extracellular stimulus response to lithium ion positive regulation of gene expression cell growth muscle filament sliding skeletal muscle fiber adaptation response to steroid hormone mesenchyme migration regulation of blood pressure response to virus vascular smooth muscle contraction glomerular mesangial cell development morphogenesis of a polarized epithelium establishment or maintenance of cell polarity regulation of transmembrane transporter activity negative regulation of protein binding adherens junction assembly apical protein localization synaptic vesicle endocytosis regulation of norepinephrine uptake protein localization to adherens junction cellular response to cytochalasin B postsynaptic actin cytoskeleton organization regulation of protein localization to plasma membrane retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation axonogenesis cell motility apoptotic process actin filament-based movement actomyosin structure organization actin-myosin filament sliding response to drug negative regulation of apoptotic process response to ethanol cardiac myofibril assembly cardiac muscle tissue morphogenesis heart contraction cardiac muscle contraction actin filament organization sarcomere organization angiogenesis positive regulation of cell migration regulation of stress fiber assembly regulation of focal adhesion assembly positive regulation of wound healing tight junction assembly protein localization to bicellular tight junction retina development in camera-type eye cellular response to electrical stimulus metabolic process actin-mediated cell contraction response to calcium ion biological_process

Actin Monoclonal Antibody (ACTN05 (C4))

Actin Antibody (MA5-11869) in ICC/IF

产品信息

MA5-11869

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

免疫电镜技术 (IM)

亲和纯化 (AP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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