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Invitrogen
Aggrecan Neo Polyclonal Antibody
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Product Details
PA1-1746
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Mouse,
Pig,
Rat
Published species
Bovine,
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C G G N(387) I T E G E(392) of human aggrecan.
Conjugate
Form
Liquid
Concentration
0.83 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-1746 detects human, rat, mouse, bovine, canine, and porcine aggrecan neo G1.
PA1-1746 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~64 kDa protein representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins.
The PA1-1746 immunogen is a synthetic peptide corresponding to residues residues C G G N(387) I T E G E(392) of human aggrecan.
Target Information
Aggrecan also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1 is a protein that is encoded by the ACAN gene. The encoded protein is an integral part of the extracellular matrix in cartilaginous tissue and it withstands compression in cartilage. The human form of the protein is 2316 amino acids long (approximately 250 kDa) and can be expressed in multiple isoforms due to alternative splicing. Aggrecan exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. The synthesis and degradation of aggrecan are being investigated for their roles in cartilage deterioration during joint injury, disease, and aging. It contains three interglobular domains (IGDs), G1, G2, and G3 that are involved in aggregation, hyaluronan binding, cell adhesion, and chondrocyte apoptosis. Aggrecan fragments from articular cartilage are released into the synovial fluid at all stages of human osteoarthritis as a result of cleavage by aggrecanase and MMP activity. Antibodies BC-3 and BC-4 can be used to detect these neo epitopes of Aggrecanase (ARGSVXXX) and MMP (XXXVDIPEN), respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aggrecan 1; aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); Aggrecan core protein; aggrecan epidermal growth factor-like domain-1; aggrecan, structural proteoglycan of cartilage; cartilage aggregating proteoglycan; Cartilage-specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan core protein 1; CSPCP; high density cartilage proteoglycan; large aggregating proteoglycan; large aggregating proteoglycan, antigen
Gene Aliases: ACAN; Agc; AGC1; AGCAN; b2b183Clo; cmd; CSPCP; CSPG1; CSPGCP; MSK16; SEDK
UniProt ID: (Bovine) P13608, (Human) Q9UCP4, (Rat) P07897, (Mouse) Q61282
Entrez Gene ID: (Dog) 403828, (Pig) 397255, (Bovine) 280985, (Human) 176, (Rat) 58968, (Mouse) 11595
cell adhesion
skeletal system development
proteolysis
central nervous system development
keratan sulfate biosynthetic process
extracellular matrix disassembly
extracellular matrix organization
keratan sulfate catabolic process
cartilage condensation
ossification
chondrocyte development
response to radiation
response to mechanical stimulus
response to gravity
response to glucose
multicellular organism aging
response to acidic pH
response to organic cyclic compound
spinal cord development
proteoglycan biosynthetic process
collagen fibril organization
negative regulation of cell migration
response to drug
chondroblast differentiation
cellular response to growth factor stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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