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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Aldolase A/B/C Antibodies

          Invitrogen

          Aldolase A/B/C Recombinant Rabbit Monoclonal Antibody (PSH01-81)

          高级验证
          查看其他2个Aldolase A/B/C抗体

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          Cite Aldolase A/B/C Recombinant Rabbit Monoclonal Antibody (PSH01-81)

          • 抗体检测数据 (12)
          • 高级验证 (1)
          Aldolase A/B/C Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Aldolase A/B/C Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 13

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          Aldolase A/B/C Antibody (MA5-56379) in ICC/IF

          Immunofluorescence analysis of Aldolase A/B/C using U-87 MG cells. The cells were fixed 4% paraformaldehyde for 20 minutes, permeabilized with 0.1% Triton™ X-100 in PBS for 5 minutes, and blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. The cells were co-labeled with Aldolase A/B/C Recombinant Rabbit Monoclonal Antibody (PSH01-81) (Product # MA5-56379, green) and beta tublin (r... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          Aldolase A/B/C Antibody in Immunocytochemistry (ICC/IF)
          Aldolase A/B/C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Aldolase A/B/C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Aldolase A/B/C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Aldolase A/B/C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Aldolase A/B/C Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Aldolase A/B/C Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Aldolase A/B/C Antibody in Western Blot (WB)
          Aldolase A/B/C Antibody in Western Blot (WB)
          Aldolase A/B/C Antibody in Western Blot (WB)
          Aldolase A/B/C Antibody in Western Blot (WB)
          Aldolase A/B/C Antibody in Western Blot (WB)
          Aldolase A/B/C Antibody
          Aldolase A/B/C Recombinant Rabbit Monoclonal Antibody (PSH01-81)

          产品信息

          MA5-56379

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:2000-1:5000
          -

          免疫组化(石蜡) (IHC (P))

          1:20,000
          -

          免疫组化(冰冻) (IHC (F))

          1:1,000
          -

          免疫细胞化学 (ICC/IF)

          1:1,000
          -
          产品规格

          种属反应

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          Expression System

          HEK293 cells

          分类

          Recombinant Monoclonal

          类型

          Antibody

          克隆号

          PSH01-81

          抗原

          Synthetic peptide within human aldolase c aa 50-100.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Protein A

          保存液

          PBS, pH 7.4, with 0.1% BSA, 40% glycerol

          内含物

          0.05% sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Wet ice

          RRID

          AB_3668608

          产品详细信息

          Positive Control: U-87 MG cell lysate, RAW264.7 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, mouse pancreas tissue lysate, rat brain tissue lysate, rat pancreas tissue lysate, U-87 MG, mouse brain tissue, rat brain tissue.

          Tissue Specificity: Group enriched (brain, retina).

          Subcellular Location: cytoskeleton, cytosol, extracellular exosome, extracellular region.

          Sequence Similarities: 100% Mouse/Rat.

          Predicted band size: 110 kDa.

          靶标信息

          Aldolases are important glycolytic cytosolic enzymes which catalyse the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate. There are three aldolase isozymes coded by three distinct genes in mammals, namely aldolases A, B, and C. Aldolase A is heavily expressed in muscle enzyme and aldolase B is heavily expressed in liver and is regarded as a liver specific enzyme. In the adult, aldolase C is the brain-specific isozyme expressed in astrocytes and a few classes of neurons, notably Purkinje cells. Appropriate antibodies to aldolase C are therefore useful to identify astrocytes in cell culture and sectioned material, and the enzyme may be over expressed in some forms of cancer.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: ALDA; ALDB; ALDC; Aldolase 1; aldolase 1, A isoform; Aldolase 2; aldolase 2, B isoform; Aldolase 3; aldolase A, fructose-bisphosphate; Aldolase B fructose-biphosphate; Aldolase B, fructose-biphosphate; aldolase B, fructose-bisphosphatase; aldolase B, fructose-bisphosphate; aldolase C, fructose-biphosphate; aldolase C, fructose-bisphosphate; Brain-type aldolase; epididymis secretory sperm binding protein Li 87p; fructoaldolase C; fructose-1,6-biphosphate triosephosphate lyase; fructose-1,6-bisphosphate triosephosphate-lyase; Fructose-bisphosphate aldolase A; Fructose-bisphosphate aldolase B; Fructose-bisphosphate aldolase C; Liver-type aldolase; Lung cancer antigen NY-LU-1; Muscle-type aldolase; Scrapie-responsive protein 2; Zebrin II

          查看更多 收起

          基因别名: AI847350; ALDA; ALDB; ALDC; ALDCAA; Aldo-1; Aldo-2; Aldo1; ALDO2; Aldo3; ALDOA; ALDOB; ALDOC; AU040929; BC016435; F16dip7; GSD12; HEL-S-87p; LIV10; RATALDCAA; RNALDOG5; Scrg2

          查看更多 收起

          UniProt ID: (Human) P04075, (Human) P05062, (Human) P09972, (Mouse) P05064, (Rat) P05065, (Mouse) Q91Y97, (Mouse) P05063, (Rat) P09117

          查看更多 收起

          Entrez Gene ID: (Human) 226, (Human) 229, (Human) 230, (Mouse) 11674, (Rat) 24189, (Mouse) 230163, (Rat) 24190, (Mouse) 11676, (Rat) 24191

          查看更多 收起

          功能
          actin binding fructose-bisphosphate aldolase activity protein binding cytoskeletal protein binding tubulin binding identical protein binding poly(A) RNA binding fructose binding cadherin binding involved in cell-cell adhesion phosphatidylcholine binding ATPase binding fructose-1-phosphate aldolase activity protease binding molecular_function catalytic activity lyase activity aldolase
          参与通路
          platelet degranulation fructose metabolic process gluconeogenesis glycolytic process ATP biosynthetic process striated muscle contraction actin filament organization regulation of cell shape fructose 1,6-bisphosphate metabolic process muscle cell cellular homeostasis protein homotetramerization canonical glycolysis cell-cell adhesion liver development NADH oxidation response to fructose response to zinc ion cellular response to extracellular stimulus positive regulation of ATPase activity cellular response to insulin stimulus response to drug response to starvation response to amino acid response to copper ion response to glucocorticoid response to cAMP fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate vacuolar proton-transporting V-type ATPase complex assembly response to interleukin-6 epithelial cell differentiation response to hypoxia response to heat response to lipopolysaccharide response to nicotine response to estrogen response to peptide hormone response to carbohydrate response to organic cyclic compound apoptotic process aging response to organonitrogen compound organ regeneration protein heterotetramerization
          It has to be done as per old AB suggested Products section.
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