Invitrogen

Amphiregulin Recombinant Rabbit Monoclonal Antibody (PSH19-73)

Name: Amphiregulin Recombinant Rabbit Monoclonal Antibody (PSH19-73)
Brand: Invitrogen
SKU: MA571399
Availability: InStock

高级验证

查看其他16个Amphiregulin抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (8)
高级验证 (4)

Amphiregulin Antibody in Immunocytochemistry (ICC/IF)

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Amphiregulin Antibody (MA571399) in ICC/IF

Immunofluorescence analysis of Amphiregulin using NR8383 cells untreated or treated with 100 nM TPA overnight then treated with 100 ng/mL LPS for 7 hours before addition of 1 μg/mL BFA for the last 3 hours. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative go... View More

Amphiregulin Antibody in Western Blot (WB)

Amphiregulin Antibody in Flow Cytometry (Flow)

Amphiregulin Antibody in Immunoprecipitation (IP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image Amphiregulin Recombinant Rabbit Monoclonal Antibody (PSH19-73)

产品信息

MA571399

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

免疫细胞化学 (ICC/IF)

1:2,000-1:5,000

流式细胞分析 (Flow)

1:5,000

免疫沉淀 (IP)

2 µg

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH19-73

抗原

Recombinant protein within human Amphiregulin aa 1-187.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.2% ProClin 950

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Control: HCT 116 treated with 100ng/mL TPA for 48 hours add 300ng/mL BFA for last 24 hours cell lysate, A549 treated with 500ng/mL Ionomycin Ca2+ Salt and 50ng/mL TPA for 6 hours add 500ng/mL BFA for last 3 hours cell lysate, C2C12 cell lysate, C2C12 treated with 100ng/mL TPA for 48 hours add 300ng/mL BFA for last 24 hours cell lysate, EL4 cell lysate, EL4 treated with treated with 500ng/mL Ionomycin Ca2+ Salt and 50ng/mL TPA for 24 hours add 500ng/mL BFA for last 20 hours cell lysate, Mouse brain tissue lysate.

Tissue Specificity: Tissue enhanced (bone marrow, placenta, urinary bladder)

Subcellular Location: Membrane.

Sequence Similarities: 73% Mouse/Rat

Predicted band size: 28 kDa.

靶标信息

Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.

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