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Invitrogen
Biotin Polyclonal Antibody
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FIGURE: 1 / 4
Biotin Antibody (31852) in ICC/IF
Nature communications 2021 -
Immobile IP 3 Rs associate with actin and KRAP. a Numbers of Ca 2+ puffs and Ca 2+ -release sites detected in EGFP-IP 3 R1 HeLa cells in the 20 s after photolysis of ci-IP 3 with or without extracellular Ca 2+ (2.5 mM BAPTA added immediately before UV flash). Mean +- s.d., 8 cells. b Mean puff amplitudes for individual cells (mean +- s.e.m., n = 8 cells). The results establish that Ca 2+ leaking across the ... View More
Product Details
31852
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Bacteria,
Chemical,
Tag
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Biotin
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.8, with 20mg/mL sucrose
Contains
0.08% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The activity measured by a solid phase binding assay is 99%.
Reconstitute using 1 mL of distilled water; product may be stored for several weeks as an undiluted liquid. After dilution, do not use for more than one day. After one year, the reconstituted liquid product should be stored frozen in small undiluted aliquots, avoiding freeze/thaw cycles.
Target Information
Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7. It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required for incorporation of carbon dioxide into organic compounds and exhibits a MW of ~244 Da.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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