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Invitrogen
Blimp-1 Monoclonal Antibody (6D3), eBioscience™
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FIGURE: 1 / 32
Blimp-1 Antibody (14-5963-82) in IHC (P)
Immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue with citrate buffer antigen retrieval, using 10 µg/mL of Anti-Human/Mouse Blimp1 Purified or 10 µg/mL of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) followed by Anti-Rat IgG Biotin, and DAB visualization.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
14-5963-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
6D3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: This 6D3 monoclonal antibody reacts with B lymphocyte-induced maturation protein-1 (Blimp-1), a transcriptional repressor expressed in B and T cells. This approximately 90-kDa protein contains five consecutive Kruppel-like zinc finger domains by which it binds DNA. In B cells, Blimp-1 promotes terminal differentiation into antibody-secreting plasma cells during a primary immune response. In addition, Blimp-1 has been implicated in germinal center formation in response to T-dependent and T-independent antigens, as well as in some diffuse large B cell lymphomas. Expressed in CD4+ and CD8+ effector/memory T cells and FoxP3+ regulatory T cells, reports have suggested possible roles for Blimp-1 in T cell homeostasis and suppression of IL-2 production. Moreover, by suppressing Th1 differentiation, Blimp-1 plays a role in T helper cell differentiation. Finally, Blimp-1 has been demonstrated to play a significant role in the terminal differentiation of effector and effector memory CD8+ T cells during viral infection.
Applications Reported: This 6D3 antibody has been reported for use in immunoblotting (WB) and immunohistology staining of paraffin-embedded tissue sections.
Applications Tested: This 6D3 antibody has been tested by western blot of reduced A20 cell lysates. For western blottiing, this antibody can be used at 1-10 µg/mL. Moreover, this antibody was tested by immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue. Antigen retrieval was performed using 10mM citrate buffer at pH 6.0. For immunohistochemistry, this antibody can be used at 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B lymphocyte induced maturation protein; B lymphocyte-induced maturation protein 1; B-lymphocyte-induced maturation protein 1; beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP 1; MGC118922; MGC118923; MGC118924; MGC118925; OTTHUMP00000016918; OTTHUMP00000202173; PR domain 1; PR domain containing 1 with ZNF domain isoform 2; PR domain containing 1, with ZNF domain; PR domain-containing protein 1; PRDI BF1; PRDI-BF1; PRDI-BF1 alpha; PRDI-BF1 beta; PRDI-binding factor-1; RP1-134E15.1; transcriptional repressor; binds specifically to the PRDI element in the promoter of the beta-interferon gene; drives the maturation of B-lymphocytes into Ig secreting cells; unnamed protein product
Gene Aliases: b2b1765Clo; BLIMP-1; BLIMP1; PRDI-BF1; ZNFPR1A1
UniProt ID: (Human) O75626, (Mouse) Q60636
Entrez Gene ID: (Human) 639, (Mouse) 12142
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
protein binding
histone methyltransferase binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
histone deacetylase binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
morphogenesis of a branching structure
kidney development
embryonic placenta development
maternal placenta development
heart valve development
cardiac septum development
ventricular septum development
regulation of transcription from RNA polymerase II promoter
germ cell development
post-embryonic development
gene expression
positive regulation of gene expression
negative regulation of gene expression
peptidyl-arginine methylation
negative regulation of B cell proliferation
negative regulation of lipopolysaccharide-mediated signaling pathway
regulation of natural killer cell differentiation
regulation of extrathymic T cell differentiation
aorta development
regulation of cell proliferation
eye photoreceptor cell development
cell fate commitment
positive regulation of B cell differentiation
artery morphogenesis
cellular developmental process
regulation of NK T cell differentiation
retinal bipolar neuron differentiation
intestinal epithelial cell development
trophoblast giant cell differentiation
coronary vasculature development
sebum secreting cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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