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Invitrogen
CBP Polyclonal Antibody
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CBP Antibody (PA1-847) in ICC/IF
Immunofluorescent analysis of CREB Binding Protein (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CREB Binding Protein polyclonal antibody (Product # PA1-847) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incub... View More
产品信息
PA1-847
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
C. elegans,
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues A(162) T S S P A T S Q T G P G I C(176) in the nuclear factor binding domain of human CBP.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1-847 detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) from human and mouse tissues and cells.
PA1-847 has been successfully used in ChIP, ICC/IF, Western blot and IHC-P procedures. By Western blot, this antibody detects an ~265 kDa protein representing CBP and a larger, unknown protein at ~400 kDa from HeLa cell lysate.
The PA1-847 immunogen is a synthetic peptide corresponding to residues A(162) T S S P A T S Q T G P G I C(176) in the nuclear factor binding domain of human CBP. The PA1-847 immunizing peptide (Cat. # PEP-052) is available for use in neutralization and control experiments.
靶标信息
CBP for CREB-binding protein is a 265 kDa nuclear protein which probably has many of the properties expected of a CREB co-activator. It shows substantial sequence homology with p300.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CREB-binding protein; Histone lysine acetyltransferase CREBBP; Protein lactyltransferas CREBBP; Protein-lysine acetyltransferase CREBBP
基因别名: AW558298; CBP; CBP/p300; CREBBP; KAT3A; p300/CBP; RSTS
UniProt ID: (Human) Q92793
Entrez Gene ID: (Human) 1387, (Mouse) 12914
core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription coactivator activity
transcriptional repressor activity, RNA polymerase II transcription factor binding
p53 binding
chromatin binding
damaged DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
histone acetyltransferase activity
signal transducer activity
protein binding
transcription factor binding
zinc ion binding
acetyltransferase activity
peptide N-acetyltransferase activity
peroxisome proliferator activated receptor binding
MRF binding
SMAD binding
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription cofactor activity
transferase activity
transferase activity, transferring acyl groups
activating transcription factor binding
metal ion binding
histone modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
response to hypoxia
stimulatory C-type lectin receptor signaling pathway
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
protein complex assembly
protein acetylation
signal transduction
Notch signaling pathway
cell proliferation
regulation of smoothened signaling pathway
viral process
histone acetylation
N-terminal peptidyl-lysine acetylation
positive regulation of type I interferon production
cellular response to UV
homeostatic process
embryonic digit morphogenesis
regulation of apoptotic process
cellular lipid metabolic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
rhythmic process
positive regulation of cell adhesion molecule production
regulation of transcription from RNA polymerase II promoter in response to hypoxia
response to interleukin-1
cell-cell adhesion
regulation of cellular response to heat
positive regulation of G1/S transition of mitotic cell cycle
positive regulation of NIK/NF-kappaB signaling
beta-catenin-TCF complex assembly
transcription, DNA-templated
transcription from RNA polymerase II promoter
germ-line stem cell population maintenance
positive regulation of sequence-specific DNA binding transcription factor activity
