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Invitrogen
CCDC53 Polyclonal Antibody
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CCDC53 Antibody (PA5-100429) in ICC/IF
Immunofluorescent analysis of CCDC53 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CCDC53 polyclonal antibody (Product # PA5-100429) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-100429
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human WASHC3(Accession Q9Y3C0), corresponding to amino acid residues V143-S193.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total CCDC53.
靶标信息
Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Coiled-coil domain-containing protein 53; WASH complex subunit 3; WASH complex subunit CCDC53
基因别名: 2900091E11Rik; 5730495F03Rik; AD-016; CCDC53; CGI-116; RGD1563761; WASHC3; x0009
UniProt ID: (Human) Q9Y3C0, (Mouse) Q9CR27
Entrez Gene ID: (Human) 51019, (Rat) 299707, (Mouse) 67282
