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Invitrogen

CCL5 (RANTES) Polyclonal Antibody

Advanced Verification
9 Published Figures
8 References
View all (29) CCL5 (RANTES) antibodies

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Product Details

P230E

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-
View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent
View 3 publications 3 publications
Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human RANTES

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Amount

500 µg

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223462

Product Specific Information

P230E targets RANTES in ELISA, and WB applications and shows reactivity with Human samples.



The P230E immunogen is recombinant human RANTES.



P230E detects RANTES which has a predicted molecular weight of approximately 8 kDa.



The P230E Rantes polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M230B (biotinylated conjugate of clone VL-1) Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.



Antibody P230E and biotinylated antibody M230B (clone VL-1) have successfully been used in combination with recombinant Rantes protein SRNTS in ELISA applications.

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; OTTHUMP00000197106; regulated upon activation, normally T-expressed, and presumably secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T cell-specific protein precursor; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228; unnamed protein product

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Gene Aliases: CCL5; D17S136E; eoCP; RANTES; SCYA5; SIS-delta; SISd; TCP228

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UniProt ID: (Human) P13501

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Entrez Gene ID: (Human) 6352

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Function(s)
phosphatidylinositol phospholipase C activity protein kinase activity cytokine activity protein binding chemokine activity phospholipase activator activity receptor signaling protein tyrosine kinase activator activity CCR1 chemokine receptor binding CCR4 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity chemokine receptor binding identical protein binding protein homodimerization activity chemokine receptor antagonist activity CCR chemokine receptor binding
Process(es)
dendritic cell chemotaxis monocyte chemotaxis regulation of chronic inflammatory response calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response immune response leukocyte cell-cell adhesion G-protein coupled receptor signaling pathway cell-cell signaling response to virus response to toxic substance positive regulation of macrophage chemotaxis positive regulation of T cell chemotaxis positive regulation of smooth muscle cell migration positive regulation of cell migration phospholipase D-activating G-protein coupled receptor signaling pathway positive regulation of TOR signaling positive regulation of cell-cell adhesion mediated by integrin response to cytokine positive regulation of homotypic cell-cell adhesion response to tumor necrosis factor chemokine (C-C motif) ligand 5 signaling pathway positive regulation of T cell proliferation neutrophil activation negative regulation by host of viral transcription cellular response to fibroblast growth factor stimulus positive regulation of viral genome replication negative regulation of viral genome replication positive regulation of innate immune response negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of G-protein coupled receptor protein signaling pathway positive regulation of cell adhesion positive regulation of translational initiation eosinophil chemotaxis macrophage chemotaxis positive regulation of smooth muscle cell proliferation epithelial cell proliferation positive regulation of epithelial cell proliferation regulation of insulin secretion regulation of T cell activation positive chemotaxis positive regulation of protein kinase B signaling positive regulation of calcium ion transport antimicrobial humoral immune response mediated by antimicrobial peptide chemokine-mediated signaling pathway negative regulation of chemokine-mediated signaling pathway negative regulation of T cell apoptotic process positive regulation of T cell apoptotic process ERK1 and ERK2 cascade cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of monocyte chemotaxis receptor signaling pathway via STAT cellular response to virus positive regulation of neuroinflammatory response positive regulation of STAT cascade negative regulation of macrophage apoptotic process positive regulation of T cell migration positive regulation of natural killer cell chemotaxis
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