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Invitrogen
CCR7 Recombinant Rabbit Monoclonal Antibody (SR36-04)
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CCR7 Antibody (MA5-31992) in ICC/IF
Immunofluorescence analysis of C-C chemokine receptor type 7 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CCR7 Recombinant Rabbit Monoclonal Antibody (SR36-04) (Product # MA5-31992) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Seco... View More
产品信息
MA5-31992
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SR36-04
抗原
Synthetic peptide within Human CCR7 aa 13-62
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: BLR2; Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; C-C CKR-7; CC chemokine receptor 7; CD197; CDw197; chemokine (C-C motif) receptor 7; chemokine (C-C) receptor 7; EBI1; EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus-induced G-protein coupled receptor 1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor
基因别名: BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; Ebi1h; EVI1
UniProt ID: (Human) P32248, (Mouse) P47774
Entrez Gene ID: (Human) 1236, (Rat) 287673, (Mouse) 12775
G-protein coupled receptor activity
C-C chemokine receptor activity
chemokine (C-C motif) ligand 19 binding
chemokine (C-C motif) ligand 21 binding
C-C motif chemokine 19 receptor activity
C-C motif chemokine 21 receptor activity
signal transducer activity
chemokine receptor activity
protein binding
myeloid dendritic cell chemotaxis
positive regulation of dendritic cell antigen processing and presentation
positive regulation of hypersensitivity
positive regulation of humoral immune response
chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
response to lipopolysaccharide
regulation of interferon-gamma production
positive regulation of interleukin-12 production
chemokine (C-C motif) ligand 19 signaling pathway
chemokine (C-C motif) ligand 21 signaling pathway
negative thymic T cell selection
regulation of interleukin-1 beta secretion
positive regulation of T cell receptor signaling pathway
chemokine-mediated signaling pathway
cellular response to cytokine stimulus
interleukin-12 secretion
positive regulation of neutrophil chemotaxis
mature conventional dendritic cell differentiation
positive regulation of dendritic cell chemotaxis
positive regulation of immunological synapse formation
positive regulation of T cell costimulation
positive regulation of glycoprotein biosynthetic process involved in immunological synapse formation
establishment of T cell polarity
positive regulation of cell-matrix adhesion
dendritic cell chemotaxis
positive regulation of cytosolic calcium ion concentration
positive regulation of actin filament polymerization
positive regulation of pseudopodium assembly
ruffle organization
response to prostaglandin E
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of cell adhesion
positive regulation of protein kinase activity
positive regulation of JNK cascade
homeostasis of number of cells
release of sequestered calcium ion into cytosol
positive regulation of filopodium assembly
positive regulation of protein kinase B signaling
positive regulation of ERK1 and ERK2 cascade
response to nitric oxide
activation of GTPase activity
lymphocyte migration into lymph node
negative regulation of leukocyte apoptotic process
positive regulation of cell motility
regulation of dendritic cell dendrite assembly
lymphocyte chemotaxis across high endothelial venule
regulation of tolerance induction to self antigen
signal transduction
activation of JUN kinase activity
positive regulation of macrophage chemotaxis
positive regulation of T cell chemotaxis
actin cytoskeleton organization
positive regulation of tumor necrosis factor production
positive regulation of T cell proliferation
positive regulation of GTPase activity
positive regulation of T-helper 1 cell differentiation
positive regulation of receptor-mediated endocytosis
lymph node development
positive regulation of interleukin-1 beta secretion
positive regulation of phagocytosis
positive regulation of thymocyte migration
