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Invitrogen
CD105 (Endoglin) Monoclonal Antibody (SN6), Functional Grade, eBioscience™
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CD105 (Endoglin) Antibody (16-1057-82) in ICC/IF
JBRA assisted reproduction 2022 -
Immunostaining of human FF cells for specific cell surface markers after 48h. (a) Red arrows show cells low positive expression of CD105, (c) negative expression of CD34. Cell nuclei were counterstained by DAPI (b, d); 20X.
产品信息
16-1057-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Cat,
Fruit fly,
Human,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
SN6
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.
Applications Reported: This SN6 antibody has been reported to have functional activity.
Applications Tested: This SN6 antibody has been tested by flow cytometric analysis of U937 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD105; CD105 antigen; Endoglin; ORW
基因别名: END; ENG; HHT1; ORW1
UniProt ID: (Human) P17813
Entrez Gene ID: (Human) 2022
transmembrane signaling receptor activity
transforming growth factor beta receptor, cytoplasmic mediator activity
type II transforming growth factor beta receptor binding
protein binding
galactose binding
glycosaminoglycan binding
type I transforming growth factor beta receptor binding
BMP binding
protein homodimerization activity
activin binding
transforming growth factor beta binding
transmembrane signal receptor
chronological cell aging
patterning of blood vessels
vasculogenesis
positive regulation of protein phosphorylation
heart looping
sprouting angiogenesis
endocardial cushion morphogenesis
cardiac ventricle morphogenesis
cardiac atrium morphogenesis
ventricular trabecula myocardium morphogenesis
cell migration involved in endocardial cushion formation
regulation of transcription, DNA-templated
cell adhesion
negative regulation of gene expression
positive regulation of pathway-restricted SMAD protein phosphorylation
regulation of transforming growth factor beta receptor signaling pathway
central nervous system vasculogenesis
negative regulation of cell migration
BMP signaling pathway
positive regulation of BMP signaling pathway
negative regulation of protein autophosphorylation
dorsal aorta morphogenesis
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
smooth muscle tissue development
artery morphogenesis
venous blood vessel morphogenesis
endocardial cushion to mesenchymal transition
positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation
