Invitrogen

CD105 (Endoglin) Monoclonal Antibody (SN6), eFluor™ 450, eBioscience™

Name: CD105 (Endoglin) Monoclonal Antibody (SN6), eFluor\u2122 450, eBioscience\u2122
Brand: Invitrogen
SKU: 48-1057-42

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64 篇参考文献

查看其他88个CD105 (Endoglin)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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CD105 (Endoglin) Antibody in Flow Cytometry (Flow)

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CD105 (Endoglin) Antibody (48-1057-42) in Flow

Staining of U937 cells with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human CD105 (Endoglin) eFluor® 450 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

CD105 (Endoglin) Antibody in Immunocytochemistry (ICC/IF)

CD105 (Endoglin) Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

CD105 (Endoglin) Antibody in Immunohistochemistry (IHC)

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产品信息

48-1057-42

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Human

已发表种属

Fruit fly, Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SN6

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11219684

产品详细信息

Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported: This SN6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SN6 antibody has been pre-titrated and tested by flow cytometric analysis of the U937 cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。