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Invitrogen
CD135 (Flt3) Monoclonal Antibody (A2F10), PE-Cyanine5, eBioscience™
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产品信息
15-1351-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
A2F10
偶联物
激发/发射光谱
568/666 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.
Applications Reported: This A2F10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD135; Fetal liver kinase 2; FL cytokine receptor; FLK-2; FLT-3; Fms-like tyrosine kinase 3; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; tyrosine-protein kinase FLT3; Tyrosine-protein kinase receptor flk-2
基因别名: B230315G04; CD135; Flk-2; Flk2; Flt-3; Flt3; Ly72; wmfl
UniProt ID: (Mouse) Q00342
Entrez Gene ID: (Mouse) 14255
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
cytokine receptor activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
ubiquitin protein ligase binding
protein complex binding
glucocorticoid receptor binding
phosphatidylinositol 3-kinase binding
transmembrane signal receptor
leukocyte homeostasis
positive regulation of protein phosphorylation
myeloid progenitor cell differentiation
lymphoid progenitor cell differentiation
pro-B cell differentiation
pro-T cell differentiation
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
negative regulation of cell proliferation
phosphorylation
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
hemopoiesis
lymphocyte differentiation
cell differentiation
B cell differentiation
common myeloid progenitor cell proliferation
negative regulation of B cell differentiation
lymphocyte proliferation
protein autophosphorylation
cellular response to cytokine stimulus
dendritic cell differentiation
