Invitrogen

CD244 Recombinant Mouse Monoclonal Antibody (eBioDM244), Alexa Fluor™ Plus 488

Name: CD244 Recombinant Mouse Monoclonal Antibody (eBioDM244), Alexa Fluor\u2122 Plus 488
Brand: Invitrogen
SKU: 740136MP488
Availability: InStock

高级验证

查看其他36个CD244抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (2)
高级验证 (1)

图: 1 / 3

CD244 Antibody (740136MP488) in Flow

Flow cytometry analysis of CD244 Recombinant Mouse Monoclonal Antibody (eBioDM244), Alexa Fluor™ Plus 488 (Product # 740136MP488) was performed using Human PBMCs. Human PBMCs were stained with CD56 (NCAM) Monoclonal Antibody (TULY56), eFluor™ 450, eBioscience™ (Product # 48-0566-42) and 2 µg of CD244 Recombinant Mouse Monoclonal Antibody (eBioDM244), Alexa Fluor™ Plus 488 (Product # 740136MP488) (Fig. b) or left unstained (Fig. a). Viable cells were used for analysis as determined by LIVE/DEAD™ Fixable Near IR (780) Viability Kit, for 633 ... View More

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

CD244 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target. CD244 Recombinant Mouse Monoclonal Antibody (eBioDM244), Alexa Fluor™ Plus 488 (Product # 740136MP488) specifically stains CD56 positive (CD56+) subset of Human PBMCs (pink histogram) while negligible CD244 staining is seen in CD56 negative (CD56-) subset of Human PBMCs (blue histogram) (Fig.b). Fig. a represents the scatter plot showing clear separation of CD56+ and CD56- subsets of Human PBMCs. {RE}

产品信息

740136MP488

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.0625-2 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

eBioDM244

偶联物

Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488

激发/发射光谱

493/518 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

50 µg

纯化类型

Protein A/G

保存液

proprietary buffer, pH 6.3-7.3

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD244 (2B4, SLAMF4) is a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via CD244 is thought to modulate NK-cell cytolytic activity. CD244 interacts with SLAMF2, causing the activation of both SLAMF4- and SLAMF2-expressing cells. Patients with systemic lupus erythmatosus have lower than normal levels of SLAMF4 expressed on their NK cells and monocytes, suggesting that SLAMF4 may play a role in the pathology of this autoimmune disease. Diseases associated with CD244 dysfunction include rheumatoid arthritis and lymphoproliferative syndrome. Alternatively spliced transcript variants encoding different isoforms of CD244 have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: alternatively spliced; CD244 molecule, natural killer cell receptor 2B4; cell surface glycoprotein; immunoglobulin superfamily; h2B4; NK cell activation inducing ligand NAIL; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NKR Nmrk; signaling lymphocytic activation molecule 4; SLAM family member 4; unnamed protein product

查看更多收起

基因别名: 2B4; NAIL; NKR2B4; Nmrk; SLAMF4

查看更多收起

UniProt ID: (Human) Q9BZW8

查看更多收起

Entrez Gene ID: (Human) 51744

查看更多收起

功能

protein binding signaling receptor activity MHC class I protein binding

参与通路

natural killer cell activation involved in immune response immune response signal transduction natural killer cell activation positive regulation of interferon-gamma production positive regulation of interleukin-8 production positive regulation of inositol phosphate biosynthetic process positive regulation of granzyme B production