Invitrogen

CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), PE-Cyanine7, eBioscience™

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产品信息

25-0299-42

应用
建议稀释比
已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test
查看14篇已发表文章 14篇已发表文章
产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

推荐的同型对照

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

TS2/16

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱 spectra

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2848300

产品详细信息

Description: The TS2/16 monoclonal antibody reacts with human CD29, also known as integrin beta 1, an approximately 130 kDa single-pass transmembrane glycoprotein. CD29 complexes with one of nine integrin alpha subunits to form the very late antigen (VLA) subfamily of adhesion molecules. Integrin heterodimers containing CD29 are involved in cell-cell and cell-matrix adhesion. CD29 is expressed broadly on lymphocytes and monocytes, with lower levels of expression on granulocytes. The TS2/16 antibody has been found to possess activating activity for beta 1 integrins.

Applications Reported: This IP26 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This IP26 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser

靶标信息

CD29, also known as ITGB1 or Integrin Subunit Beta 1, is a 110 kDa cell surface glycoprotein widely expressed by various cells, including all leukocytes. It forms non-covalently linked heterodimers with at least six different alpha integrins (CD49a-f), creating VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers mediate a range of cellular responses, including adhesion, trafficking, proliferation, and differentiation. CD29-containing integrins bind to extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin. Some integrins also interact with cellular receptors like VCAM-1 and MadCAM-1. CD29 plays a crucial role in immune cell trafficking from blood to tissue, particularly during leukocyte emigration and extravascular migration. It is involved in regulating tissue damage and disease symptoms related to inflammatory bowel disease. In the presence of anti-tumor drugs, decreased expression of ITGB1 correlates with multidrug resistance in tumor cells. ITGB1-dependent mechanisms enhance cytokine secretion in human airway smooth muscle in response to IL-1beta, facilitated by fibronectin and type I collagen. More than eight beta subunits with numerous splice variant isoforms have been identified in mammals, including two major forms: beta1A and beta1D. Beta1A is present in all tissues except cardiac and skeletal muscle, which express the beta1D variant.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD29; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; integrin beta 1; Integrin beta-1; Integrin beta1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITBG1D; very late activation protein, beta polypeptide; VLA-4 subunit beta

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基因别名: CD29; FNRB; GPIIA; ITGB1; MDF2; MSK12; VLA-BETA; VLAB

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UniProt ID: (Human) P05556

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Entrez Gene ID: (Human) 3688

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功能
virus receptor activity glycoprotein binding fibronectin binding protease binding actin binding integrin binding protein binding coreceptor activity protein kinase binding protein domain specific binding C-X3-C chemokine binding protein complex binding peptide binding laminin binding metal ion binding protein heterodimerization activity cell adhesion molecule binding alpha-actinin binding collagen binding involved in cell-matrix adhesion cadherin binding involved in cell-cell adhesion integrin
参与通路
G1/S transition of mitotic cell cycle in utero embryonic development cell fate specification tissue homeostasis cell migration involved in sprouting angiogenesis cellular calcium ion homeostasis cellular defense response homophilic cell adhesion via plasma membrane adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion calcium-independent cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway regulation of G-protein coupled receptor protein signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration visual learning regulation of collagen catabolic process positive regulation of cell-substrate adhesion positive regulation of neuron projection development response to activity cell migration formation of radial glial scaffolds B cell differentiation extracellular matrix organization positive regulation of cell migration negative regulation of cell projection organization cell-substrate adhesion receptor internalization protein transport within lipid bilayer cell-cell adhesion mediated by integrin heterotypic cell-cell adhesion response to gonadotropin negative regulation of Rho protein signal transduction response to drug positive regulation of apoptotic process stress fiber assembly positive regulation of MAPK cascade positive regulation of GTPase activity sarcomere organization negative regulation of neuron differentiation positive regulation of endocytosis viral entry into host cell mesodermal cell differentiation axon extension dendrite morphogenesis positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response leukocyte migration leukocyte tethering or rolling regulation of cell cycle cardiac muscle cell differentiation maternal process involved in female pregnancy bicellular tight junction assembly cellular response to mechanical stimulus cellular response to vitamin D cellular response to low-density lipoprotein particle stimulus cellular response to ionizing radiation positive regulation of establishment of protein localization to plasma membrane negative regulation of anoikis
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