Invitrogen

CD309 (FLK1) Monoclonal Antibody (Avas12a1), PE, eBioscience™

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52 篇参考文献
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产品信息

12-5821-82

应用
建议稀释比
已发表文章

流式细胞分析 (Flow)

0.5 µg/test
查看51篇已发表文章 51篇已发表文章

其他PubMed (Misc)

-
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产品规格

种属反应

Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

推荐的同型对照

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

Avas12a1

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱 spectra

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_465967

产品详细信息

Description: The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.

Applications Reported: The Avas12a1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

生物信息学

蛋白别名: CD309; CD309 antigen; Fetal liver kinase 1; FLK-1; Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

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基因别名: 6130401C07; Flk-1; Flk1; Kdr; Krd-1; Ly73; sVEGFR-2; VEGFR-2; VEGFR2

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UniProt ID: (Mouse) P35918

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Entrez Gene ID: (Mouse) 16542

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功能
nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity integrin binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding vascular endothelial growth factor binding transmembrane signal receptor
参与通路
angiogenesis ovarian follicle development vasculogenesis response to hypoxia positive regulation of protein phosphorylation regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation lymph vessel development cell migration involved in sprouting angiogenesis positive regulation of mesenchymal cell proliferation endocardium development endothelium development endochondral bone growth protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cytosolic calcium ion concentration multicellular organism development positive regulation of cell proliferation regulation of cell shape male gonad development positive regulation of endothelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling phosphorylation cell migration peptidyl-tyrosine phosphorylation hemopoiesis cell differentiation lung development positive regulation of cell migration positive regulation of BMP signaling pathway positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of TOR signaling embryonic hemopoiesis calcium-mediated signaling using intracellular calcium source cellular response to vascular endothelial growth factor stimulus peptidyl-tyrosine autophosphorylation vascular endothelial growth factor signaling pathway response to drug negative regulation of apoptotic process surfactant homeostasis positive regulation of MAPK cascade negative regulation of neuron apoptotic process cell fate commitment endothelial cell differentiation regulation of endothelial cell differentiation positive regulation of angiogenesis positive regulation of vasodilation protein autophosphorylation vascular endothelial growth factor receptor signaling pathway positive regulation of long-term neuronal synaptic plasticity lung alveolus development cell maturation branching morphogenesis of an epithelial tube neuron projection morphogenesis positive regulation of epithelial cell proliferation positive regulation of calcium-mediated signaling positive regulation of positive chemotaxis positive regulation of nitric-oxide synthase biosynthetic process positive regulation of focal adhesion assembly calcium ion homeostasis blood vessel endothelial cell differentiation positive regulation of ERK1 and ERK2 cascade regulation of hematopoietic progenitor cell differentiation negative regulation of endothelial cell apoptotic process positive regulation of vasculogenesis
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