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Invitrogen
CD324 (E-Cadherin) Monoclonal Antibody (DECMA-1), Biotin, eBioscience™
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CD324 (E-Cadherin) Antibody (13-3249-82) in Flow
Staining of the MDCK cell line with 0.125 µg of Rat IgG1 kappa Isotype Control Biotin (Product # 13-4301-82) (blue histogram) or 0.125 µg of Anti-Human CD324 (E-Cadherin) Biotin (purple histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
产品信息
13-3249-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human,
Mouse
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
DECMA-1
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton.
Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintenance of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers.
E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided.
The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells.
Applications Reported: This DECMA-1 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This DECMA-1 antibody has been tested by flow cytometric analysis of the MDCK cell line. Optimal staining is achieved by intracellular staining as protein turnover can result in variable surface staining. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin e; Cadherin-1; cadherin-E; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Ecadherin; Epithelial cadherin; Uvomorulin
基因别名: AA960649; Arc-1; Cadherin-1; CD324; CDH1; CDHE; E-cad; ECAD; L-CAM; LCAM; Um; UVO; Uvomorulin
UniProt ID: (Dog) F1PAA9, (Human) P12830, (Mouse) P09803
Entrez Gene ID: (Dog) 442858, (Human) 999, (Mouse) 12550
protein binding
beta-catenin binding
ankyrin binding
GTPase activating protein binding
identical protein binding
gamma-catenin binding
glycoprotein binding
calcium ion binding
cell adhesion molecule binding
cadherin binding involved in cell-cell adhesion
protein phosphatase binding
protein domain specific binding
metal ion binding
cadherin
homophilic cell adhesion via plasma membrane adhesion molecules
regulation of gene expression
negative regulation of cell-cell adhesion
negative regulation of cell migration
adherens junction organization
positive regulation of protein import into nucleus
positive regulation of transcription, DNA-templated
cellular response to lithium ion
cellular response to indole-3-methanol
protein localization to plasma membrane
synapse assembly
response to toxic substance
single organismal cell-cell adhesion
pituitary gland development
extracellular matrix disassembly
extracellular matrix organization
neuron projection development
response to drug
positive regulation of transcription factor import into nucleus
establishment of protein localization to plasma membrane
in utero embryonic development
trophectodermal cell differentiation
epithelial cell morphogenesis
cell adhesion
sensory perception of sound
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
protein metabolic process
regulation of protein localization
regulation of water loss via skin
regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of epithelial cell proliferation
negative regulation of epithelial cell proliferation
protein homooligomerization
intestinal epithelial cell development
salivary gland cavitation
regulation of branching involved in salivary gland morphogenesis
bicellular tight junction assembly
cellular response to amino acid stimulus
negative regulation of canonical Wnt signaling pathway
cochlea development
regulation of protein localization to cell surface
regulation of neuron migration
