Invitrogen

CD49e (Integrin alpha 5) Monoclonal Antibody (eBioHMa5-1 (HMa5-1)), FITC, eBioscience™

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产品信息

11-0493-81

应用
建议稀释比
已发表文章

流式细胞分析 (Flow)

1 µg/test
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产品规格

种属反应

Mouse, Rat

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Armenian hamster / IgG

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Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

eBioHMa5-1 (HMa5-1)

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱 spectra

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1234968

产品详细信息

Description: The eBioHMalpha5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin alpha5 or VLA-5A. CD49e is a member of the integrin alpha chain family, and binds with CD29 (integrin beta1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The eBioHMalpha5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates. Furthermore, HMalpha5-1 injected into mice subcutaneously, along with anti-CD49d and anti-CD61, is able to inhibit passive cutaneous anaphylaxis.

Applications Reported: This eBioHMa5-1 (HMa5-1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioHMa5-1 (HMa5-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

生物信息学

蛋白别名: CD49 antigen-like family member E; CD49e; fibronectin receptor alpha polypeptide; Fibronectin receptor subunit alpha; integrin alpha 5 (fibronectin receptor alpha); Integrin alpha-5; Integrin alpha-F; VLA-5

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基因别名: Cd49e; Fnra; Itga5; Itga5_retired; VLA5

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UniProt ID: (Mouse) P11688

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Entrez Gene ID: (Mouse) 16402, (Rat) 315346

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