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Invitrogen
CD8b Polyclonal Antibody
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FIGURE: 1 / 1
CD8b Antibody (PA5-109499) in WB
Western blot analysis of CD8b in extracts of various cell lines. Samples were incubated with CD8b Polyclonal antibody (Product # PA5-109499) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-109499
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 22-170 of human CD8B (NP_757362.1).
Conjugate
Form
Liquid
Concentration
0.75 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: LQQTPAYIKV QTNKMVMLSC EAKISLSNMR IYWLRQRQAP SSDSHHEFLA LWDSAKGTIH GEEVEQEKIA VFRDASRFIL NLTSVKPEDS GIYFCMIVGS PELTFGKGTQ LSVVDFLPTT AQPTKKSTLK KRVCRLPRPE TQKGPLCSP
Target Information
The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD8; CD8 antigen 37 kDa chain; CD8 antigen beta-chain; CD8 antigen, beta chain; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8a; CD8alpha; CD8b; CD8beta; Leu-2; Lymphocyte antigen 3; MAL; OX-8 membrane antigen; p32; T lymphocyte surface glycoprotein beta chain; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-3
Gene Aliases: CD8B; CD8B1; LEU2; Ly-3; Ly-C; LY3; Lyt-3; LYT3; P37
UniProt ID: (Human) P10966, (Rat) P05541, (Mouse) P10300
Entrez Gene ID: (Human) 926, (Rat) 24931, (Mouse) 12526
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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