Invitrogen

CLIF Polyclonal Antibody

Name: CLIF Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-114384

查看其他3个CLIF抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

图: 1 / 3

CLIF Antibody (PA5-114384) in WB

Western blot analysis of CLIF in the following samples: Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat ovary tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse testis tissue lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) and washed with TBS-0.1% Tween (3 times, 5 minutes/wash... View More

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Immunogen Image CLIF Polyclonal Antibody

产品信息

PA5-114384

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human ARNTL2 recombinant protein (Position: E461-T575).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2884840

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human PC-3 whole cell, human A549 whole cell, human HepG2 whole cell, rat ovary tissue, mouse lung tissue, mouse testis tissue. Flow: A431 cell, HL-60 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (PER, ARNT, SIM) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels, exposure to certain environmental pollutants, and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian CLOCK protein, the structurally related MOP4, and hypoxia-inducible factors, such as HIF1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors, this protein is coexpressed in regions of the brain such as the thalamus, hypothalamus, and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: aryl hydrocarbon receptor nuclear translocator-like 2; Aryl hydrocarbon receptor nuclear translocator-like protein 2; Basic helix-loop-helix ARNT-like protein 2; Basic-helix-loop-helix-PAS protein MOP9; bHLHe6; Brain and muscle ARNT-like 2; brain and muscle Arnt-like protein 2 variant d; brain-muscle-ARNT-like protein 2; Class E basic helix-loop-helix protein 6; CLIF; CYCLE-like factor; Member of PAS protein 9; MGC149671; MGC149672; PAS domain-containing protein 9; transcription factor BMAL2

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基因别名: 4632430A05Rik; Arnt4; ARNTL2; BHLHE6; BMAL2; CLIF; MOP9; PASD9

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UniProt ID: (Human) Q8WYA1, (Mouse) Q2VPD4

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Entrez Gene ID: (Human) 56938, (Mouse) 272322, (Rat) 362464

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功能

transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein dimerization activity E-box binding DNA binding signal transducer activity protein binding molecular_function basic helix-loop-helix transcription factor

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