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Invitrogen
CR16 Polyclonal Antibody
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CR16 Antibody (PA5-144045) in WB
Western blot analysis of WIPF3 using WIPF3 antibody (Product # PA5-144045). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human K562 whole cell lysates; Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was ... View More
产品信息
PA5-144045
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to a sequence of human WIPF3 (EDFPPPDEYKPCQKIYPSKIPR).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human 293T whole cell. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
May be a regulator of cytoskeletal organization. May have a role in spermatogenesis. Target protein binds SH3 domains; may play a role in MAP kinase mediated signal transduction.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Corticosteroids and regional expression protein 16 homolog; FLJ36931; WAS/WASL interacting protein family, member 3; WAS/WASL-interacting protein family member 3
基因别名: CR16; WIPF3
UniProt ID: (Human) A6NGB9
Entrez Gene ID: (Human) 644150
actin cortical patch assembly
endocytosis
multicellular organism development
spermatogenesis
regulation of cell shape
actin filament-based movement
cell differentiation
Fc-gamma receptor signaling pathway involved in phagocytosis
positive regulation of actin nucleation
actin cortical patch localization
