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Calmodulin 2 Polyclonal Antibody
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FIGURE: 1 / 1
Calmodulin 2 Antibody (PA5-121166) in WB
Western blot analysis of Calmodulin 2 in extracts of various cell lines. Samples were incubated with Calmodulin 2 Polyclonal antibody (Product # PA5-121166) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-121166
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-149 of human CALM2 (NP_001734.1).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.08 mg/mL
Amount
108 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: NIH/3T3, MCF7, BT-474, NCI-H460, Mouse brain. The target is usually found in the following locations: Cytoplasm, cytoskeleton, spindle, spindle pole.
Immunogen sequence: MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK
Target Information
Calmodulin 2 (CALM2) is one of the three human genes encoding calmodulin, a highly conserved calcium-binding messenger protein critical for mediating various cellular processes. Calmodulin proteins are composed of two pairs of EF-hand motifs located at both the N- and C-termini, connected by a flexible central linker. Each EF-hand motif binds calcium ions, with the C-terminus possessing two high-affinity Ca2+ binding sites, while the N-terminus contains two low-affinity sites. Upon binding calcium, calmodulin undergoes a conformational change that enables it to interact with and regulate numerous target proteins, including kinases, phosphatases, ion channels, and other enzymes. Specifically, calcium/calmodulin (CaM) signaling pathways are involved in critical cellular functions such as muscle contraction, neurotransmitter release, and cellular growth. The interaction of CALM2 with different targets is essential for translating transient changes in intracellular calcium levels into specific cellular responses, making it a pivotal player in calcium signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CALM2; calmodulin; calmodulin 2 (phosphorylase kinase, delta); Calmodulin-1; Calmodulin-3; caM; LP7057 protein; phosphorylase kinase delta; phosphorylase kinase subunit delta; phosphorylase kinase subunit delta 2; prepro-calmodulin 2; unnamed protein product
Gene Aliases: 1500001E21Rik; CALM; CALML2; caM; CAM1; Cam2; CAM3; CAMC; CAMII; CAMIII; LQT15; PHKD; PHKD2
UniProt ID: (Human) P0DP23, (Mouse) P0DP27
Entrez Gene ID: (Human) 805, (Mouse) 12314
calcium channel regulator activity
calcium ion binding
protein binding
adenylate cyclase binding
adenylate cyclase activator activity
calcium channel inhibitor activity
protein kinase binding
titin binding
protein serine/threonine kinase activator activity
ion channel binding
calcium-dependent protein binding
protein phosphatase activator activity
transporter inhibitor activity
protein domain specific binding
nitric-oxide synthase regulator activity
type 3 metabotropic glutamate receptor binding
phosphatidylinositol 3-kinase binding
nitric-oxide synthase binding
G2/M transition of mitotic cell cycle
response to amphetamine
regulation of heart rate
detection of calcium ion
endocytosis
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
autophagosome membrane docking
regulation of cytokinesis
cellular response to interferon-beta
positive regulation of JAK-STAT cascade
regulation of calcium-mediated signaling
response to corticosterone
response to calcium ion
establishment of localization in cell
regulation of cardiac muscle contraction
negative regulation of ryanodine-sensitive calcium-release channel activity
cellular response to interferon-gamma
calcineurin-mediated signaling
regulation of cardiac muscle cell action potential
organelle localization by membrane tethering
presynaptic endocytosis
regulation of synaptic vesicle endocytosis
regulation of cell communication by electrical coupling involved in cardiac conduction
negative regulation of calcium ion export across plasma membrane
regulation of synaptic vesicle exocytosis
G-protein coupled receptor signaling pathway
substantia nigra development
long-term synaptic potentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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