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Invitrogen
Cd8a Polyclonal Antibody
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Cd8a Antibody (PA5-114369) in IHC (P)
Immunohistochemistry analysis of Cd8a in paraffin-embedded rat spleen tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in Cd8a polyclonal antibody (Product # PA5-114369) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.
Product Details
PA5-114369
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived rat CD8 alpha/Cd8a recombinant protein (Position: Q27-Q114).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat thymus issue, rat thymus issue. IHC: rat spleen tissue, rat thymus tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD8 antigen 32 kDa chain; CD8 antigen 37 kDa chain; CD8 antigen alpha-chain; CD8 antigen beta-chain; CD8 antigen, alpha chain; CD8 antigen, alpha-chain; CD8 antigen, beta chain; CD8 antigen, beta-chain; CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; OX-8 membrane antigen; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain
Gene Aliases: Cd8a; Cd8b; Cd8b1
UniProt ID: (Rat) P07725, (Rat) P05541
Entrez Gene ID: (Rat) 24930, (Rat) 24931
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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