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Invitrogen
ChAT Polyclonal Antibody
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图: 1 / 2
ChAT Antibody (PA5-77838) in ICC/IF
Immunofluorescent analysis of ChAT in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ChAT polyclonal antibody (Product # PA5-77838) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain B) Phalloidin Texas Red F-Actin stain C) Choline Acetyltransferase Antibody D) Merged image... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-77838
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
A 1:1,000 dilution of PA5-77838 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.|Predicted molecular weight at approximately 82.5kDa. Observed molecular weights between 68-70kDa.
靶标信息
ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: acetyl CoA; CHOACTase; choline acetylase; Choline O-acetyltransferase
基因别名: B230380D24Rik; CHAT
Entrez Gene ID: (Mouse) 12647
chemical synaptic transmission
neuromuscular synaptic transmission
muscle organ development
establishment of synaptic specificity at neuromuscular junction
memory
rhythmic behavior
adult walking behavior
acetylcholine biosynthetic process
dendrite development
neuron differentiation
neurotransmitter biosynthetic process
rhythmic excitation
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